Skin Toxicity

PHARM 304 LAB

Acute Dermal Toxicity

adverse effects occurring within a short time of dermal application of a single dose of a test substance

Dose

amount of test substance applied

LD50 (median lethal dose) dermal

statistically derived single dose of a substance that can be expected to cause death in 50 percent of treated animals when applied to the skin

Dosage

• general term comprising the dose

• its frequency and the duration of dosing

Dosage-response

relationship between the dose and the proportion of a population sample showing a defined effect

Dose-effect

relationship between the dose and the magnitude of a defined biological effect either in an individual or in a population sample.

200-300 g

RAT weight range

2-3 g

RABBIT weight range

350-450 g

GUINEA PIG weight range

2000 mg/kg

body weight for LIMIT TEST

14

days for OBSERVATION PERIOD