ch17&16

These flashcards cover essential concepts discussed in the lecture about biotechnology, molecular cloning, and genetic engineering.

The ultimate goal of using microorganisms, cells, or components is for __________ benefit.

human

CRISPR Cas9 allows for __________ gene deletions and insertions.

targeted

The guide RNA plays a critical role in identifying the __________ to be modified in the CRISPR Cas9 system.

target sequence

A __________ is a molecule that allows the insertion of a gene into a bacterium.

plasmid

Recombinant DNA is created from DNA of __________ different organisms.

two

A bacterium that has incorporated a gene from another organism is called a __________ organism.

transgenic

To select cells that have taken up a vector, we can use the __________ resistant gene present in the vector.

ampicillin

The purpose of blue white screening is to determine if the lac z gene is __________.

interrupted

Transformation is the process by which a cell takes up __________ DNA from the environment.

naked

Eukaryotic cells typically require __________ to incorporate foreign DNA into their genome.

transfection

In synthetic biology, cells are viewed as __________ made of different models.

modular

The primary metabolites are produced during the __________ growth phase of microorganisms.

exponential

Secondary metabolites are typically produced during the __________ phase of growth.

stationary

The phase of processing that involves the preparation culture for fermentation is known as __________ processing.

upstream

After fermentation, the processes that involve harvesting and purifying the product are called __________ processing.

downstream

Selection of the cells that have taken up the vector based on antibiotic resistance is known as __________.

selection

Blue colonies in blue white screening indicate the presence of an __________ plasmid.

intact lac z

In industrial microbiology, microorganisms are used to produce __________ valuable products.

commercially

A key tool for gene editing that allows modification of DNA sequences is __________.

CRISPR

To enhance efficiency in bioconversion, genetic manipulation techniques can be applied such as __________ engineering.

metabolic

One of the common eukaryotic cells used in recombinant DNA technology is __________.

yeast