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Autotroph
Makes its own food (e.g., plants via photosynthesis)
Heterotroph
Gets energy by consuming other organisms
Biochemical pathway
Series of chemical reactions in a cell
Aerobic
Requires oxygen
Anaerobic
Does NOT require oxygen
Cellular respiration
Process of breaking down glucose to make ATP
Fermentation
Anaerobic process that produces ATP without oxygen
Cellular Respiration Equation
C6H12O6+6O2→6CO2+6H2O+ATP
Mitochondria Structure
-Outer membrane
-Inner membrane (folded into cristae)
-Matrix (inside space)
Think (O.I.M.)
Location of CO₂ produced
Krebs cycle (matrix)
ETC Location
Inner mitochondrial membrane
3 Stages of Cellular Respiration
1.Glycolysis
L.ocation-Cytoplasm
R.eactant-Glucose
P.roducts-2 Pyruvate, ATP,NADH
2.Krebs Cycle
L-Matrix
R-Acetyl CoA
P- CO2,ATP,NADH,FADH2
3.ETC
L-Inner Membrane
R-NADH,FADH2,O2
P-ATP,H2O
ATP Production
Total per glucose: ~36-38 ATP
Glycolysis: 2 ATP
Krebs: 2 ATP
ETC: 32-34 ATP
Fermentation Types
1.Lactic Acid
Example-Muscle Cells
Product-Lactate
ATP-2
2. Alcoholic
E-Yeast
P-Ethanol + CO2
ATP-2
ATP Hydrolysis
ATP broken down→ ADP + releases energy
ETC Final Electron Acceptor
Oxygen (O₂)
Anabolism vs Catabolism
Anabolism: builds molecules (uses energy)
Catabolism: breaks down molecules (releases energy)
Photon
Light particle
autotroph
An organism that makes its own food
electromagnetic spectrum
Visible at 1 end and Gamma at the other end
Pigment
Chlorophyll (Light-absorbing molecule)
Reaction center
Chlorophyll molecule where electrons are excited
Stoma (Stomata)
Opening for gas exchange!
minute pores (nostril) in the epidermis of the leaf or stem of a plant, forming a slit of variable width that allows movement of gases in and out of the intercellular spaces.
Photosynthesis Equation
6CO2+6H2O+light→C6H12O6+6O2
Know the wavelength range of visible light
R.O.Y.G.B.I.V.
Absorbed
taken in
Reflected
bounced off (Color we see)
Transmitted
Passes through
Pigments & Absorption
Chlorophyll a: absorbs blue & red (reflects green)
Chlorophyll b: similar but slightly different range
Carotenoids: absorb blue/green (reflect yellow/orange)
Reaction Center Pigment
Chlorophyll a
Chloroplast Structure
1.Thylakoid (light reactions)
2.Stroma (Calvin cycle)
Photosynthesis Stages
1.Light-DEpendent
-Location: Thylakoid
-Inputs: Light,H20
-Outputs: O2,ATP,NADPH
2.Light-INDEpendent (Calvin Cycle)
-L: Stroma
-I: CO2,ATP,NADPH
-O: Glucose
Photosystems: which one was DISCOVERED/FOUND first?
PSI was DISCOVERED FIRST...but PSII came first but
Electron Flow
-PSII gets electrons from water
-PSI gets electrons from PSII
Photorespiration
Occurs when Rubisco binds O₂ instead of CO₂ → wastes energy
Apoptosis
programmed cell death
Homeostasis
stable internal conditions
Feedback loop
Response system that regulate processes
Why Cells Communicate
1.Coordinate functions
2.Respond to environment
3.Maintain homeostasis
Types of Communication
1.Direct contact (gap junctions/plasmodesmata)
2.Local signaling (paracrine, synaptic)
3.Long-distance (hormones/endocrine)
Cell Junctions
Animal: gap junctions
Plant: plasmodesmata
3 Stages of Signaling
1.Reception - signal binds receptor
2.Transduction - signal converted inside cell
3.Response - cell reacts
Feedback Loops
Negative feedback stabilizes systems
Apoptosis Role
Removes damaged or unnecessary cells
Genome
ALL DNA
Gene
DNA segment coding for protein
Chromosome
Condensed DNA
Chromatin
loose DNA
Binary fission
a simple form of asexual reproduction where a single parent cell divides into two identical daughter cells, Prokaryotic division
Homologous chromosomes
same genes, Different parents
Sister chromatids
Identical copies of a chromosome
Daughter chromosomes
a single-stranded chromosome that results from the separation of sister chromatids during cell division separated chromatids
Somatic cells
all the "body cells" that make up your skin, organs, bones, blood, and connective tissue, excluding reproductive cells (sperm and eggs)
Body Cells
Gametes
Sex Cells
Centromere
Chromosome center
Kinetochore
protein attachment site
Spindle fibers
microtubules
Centrosome/Centriole
Organize spindle
Eukaryotic vs Prokaryotic Genome
Eukaryotes: multiple linear chromosomes
Prokaryotes: single circular chromosome
Chromosome Composition
60% protein + 40% DNA
Interphase
G1: growth
S: DNA replication
G2: prep
M Phase
where the condensed DNA is aligned and then separated
:Prophase → Metaphase → Anaphase → Telophase
:Cytokinesis
Longest Phase
Interphase
Chromosome Comparisons
-Homologous ≠ identical
- Sister chromatids = identical
-Daughter chromosomes = separated chromatids
Chromosome Movement
Spindle fibers pull chromosomes apart
Spindle Fibers
Made of microtubules (protein)
Microtubule Shortening
Shortens at chromosome end
Cytokinesis
Animal: cleavage furrow
Plant: cell plate
Mitosis Results
1 diploid → 2 diploid cells
Examples:
Human (46) → 2 cells with 46
Dog (78) → 2 cells with 78
Diploid (2n)
two sets
Haploid (n)
one set
Chiasma
crossover point
is the X-shaped point of contact where non-sister chromatids of homologous chromosomes cross over and exchange genetic material during meiosis
Meiosis Outcome
1 diploid → 4 haploid cells
Examples:
Human (46) → 4 cells with 23
Mitosis
Divisions: 1
Cells produced: 2
Chromosome #: same
Variation: None
Meiosis
Divisions: 2
Cells produced: 4
Chromosome #: half
Variation: High
Human Life Cycle
Mitosis → somatic cells (diploid)
Meiosis → gametes (haploid)
Reproduction
Sexual: 2 parents, variation
Asexual: 1 parent, identical
Meiosis Divisions
Meiosis I: separates homologous chromosomes
Meiosis II: separates sister chromatids
Meiosis I
Interphase I, Prophase I, Metaphase I, Anaphase I, Telophase I, Cytokinesis = 2 cells
Meiosis II
Same as 1 but with number 2 at the end of each step... Cytokinesis = 4 cells
Describe the events in Meiosis I
Prophase I (crossing over)
Metaphase I (pairs align)
Anaphase I (separate pairs/going opposite poles)
Telophase I (cytoplasm dividing)
Describe the events in Meiosis II
Same but "II" instead
Metaphase Alignment
Mitosis: single file
Meiosis I: homologous pairs
Where does Synapsis take place?
Prophase 1
Where does Tetrads take place?
Prophase 1
Where does Crossing over take place?
Prophase 1
List contributions to genetic variation in sexually reproducing organisms (why are all gametes different?)
-Crossing over
- Independent assortment
- Random fertilization
Gametogenesis
1.Spermatogenesis: 4 sperm gametes
2.Oogenesis: 1 egg + polar bodies