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MICR 2 LAB: Exercise 1 - Microscope
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36 Terms
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1
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First person to experiment microorganisms "animacules"; Father of Microbiology
Antonie Van Leeuwenhoek
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Inventor of compound microscope
Galileo Galilei
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Coined the name "Microscope"
Giovanni Faber
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Published Micrographia; Cell theory
Robert Hooke
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Coined the term "Mycology"
Miles Joseph Berkley (1836)
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Most common method used for detection and characterization of microorganisms
Microscopy
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Equipment used in Microscopy
Microscope
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Part of a microscope that controls the amount of light reaching the specimen
Iris Diaphragm
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Part of a microscope that gathers light from the illuminator and focuses it into a light cone towards the specimen
Condenser
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Term for the hole of a microscope stage
Aperture
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Type of Microscope that is most commonly used and forms a dark image against a bright background
Bright field microscope
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Type of Microscope that permits detailed examination of internal structures of living organisms
Phase contrast microscope
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Type of Microscope that involves the excitation of fluorochromes by light to stain microorganisms
Fluorescent microscope
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Type of Microscope that forms a light image against a dark background
Dark field microscope
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Type of Microscope that uses electrons instead of light to visualize small objects
Electron microscope
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The ability of a microscope to visualize fine details and is used to distinguish two small objects at a specific distance apart
Resolution
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Magnification of Scanner objective
4x
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Magnification of Ocular
10x
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Magnification of LPO
10x
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Magnification of HPO
40x
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Magnification of OIO
100x
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Total Magnification of Scanner
40x
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Total Magnification of LPO
100x
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Total Magnification of HPO
400x
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Total Magnification of OIO
1000x
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Total Magnification used to view detailed fungal structures
1000x
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Substance or fluid used in OIO that has the same refractive index as glass
Cedar wood oil
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Term for when objectives that can be changed with minimal or no refocusing
Parfocal
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Most commonly used illumination in microscope
Tungsten Halogen lamp
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Term for the distance between the nosepiece opening to the top edge of the observation tubes wherein the eyepieces are inserted/mounted
Mechanical tube length
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Term for the distance between the objective lens and the surface of the specimen
Working distance (mm)
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Working distance of Scanner
27\.8 mm
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Working distance of LPO
8\.0 mm
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Working distance of HPO
0\.6 mm
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Working distance of OIO
0\.13 mm
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Disinfectant used for Microscope
70% Ethyl alcohol