First person to experiment microorganisms "animacules"; Father of Microbiology
Antonie Van Leeuwenhoek
Inventor of compound microscope
Galileo Galilei
Coined the name "Microscope"
Giovanni Faber
Published Micrographia; Cell theory
Robert Hooke
Coined the term "Mycology"
Miles Joseph Berkley (1836)
Most common method used for detection and characterization of microorganisms
Microscopy
Equipment used in Microscopy
Microscope
Part of a microscope that controls the amount of light reaching the specimen
Iris Diaphragm
Part of a microscope that gathers light from the illuminator and focuses it into a light cone towards the specimen
Condenser
Term for the hole of a microscope stage
Aperture
Type of Microscope that is most commonly used and forms a dark image against a bright background
Bright field microscope
Type of Microscope that permits detailed examination of internal structures of living organisms
Phase contrast microscope
Type of Microscope that involves the excitation of fluorochromes by light to stain microorganisms
Fluorescent microscope
Type of Microscope that forms a light image against a dark background
Dark field microscope
Type of Microscope that uses electrons instead of light to visualize small objects
Electron microscope
The ability of a microscope to visualize fine details and is used to distinguish two small objects at a specific distance apart
Resolution
Magnification of Scanner objective
4x
Magnification of Ocular
10x
Magnification of LPO
10x
Magnification of HPO
40x
Magnification of OIO
100x
Total Magnification of Scanner
40x
Total Magnification of LPO
100x
Total Magnification of HPO
400x
Total Magnification of OIO
1000x
Total Magnification used to view detailed fungal structures
1000x
Substance or fluid used in OIO that has the same refractive index as glass
Cedar wood oil
Term for when objectives that can be changed with minimal or no refocusing
Parfocal
Most commonly used illumination in microscope
Tungsten Halogen lamp
Term for the distance between the nosepiece opening to the top edge of the observation tubes wherein the eyepieces are inserted/mounted
Mechanical tube length
Term for the distance between the objective lens and the surface of the specimen
Working distance (mm)
Working distance of Scanner
27.8 mm
Working distance of LPO
8.0 mm
Working distance of HPO
0.6 mm
Working distance of OIO
0.13 mm
Disinfectant used for Microscope
70% Ethyl alcohol