MICR 2 LAB: Exercise 1 - Microscope

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First person to experiment microorganisms "animacules"; Father of Microbiology

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36 Terms

1

First person to experiment microorganisms "animacules"; Father of Microbiology

Antonie Van Leeuwenhoek

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2

Inventor of compound microscope

Galileo Galilei

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3

Coined the name "Microscope"

Giovanni Faber

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4

Published Micrographia; Cell theory

Robert Hooke

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5

Coined the term "Mycology"

Miles Joseph Berkley (1836)

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6

Most common method used for detection and characterization of microorganisms

Microscopy

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7

Equipment used in Microscopy

Microscope

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8

Part of a microscope that controls the amount of light reaching the specimen

Iris Diaphragm

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9

Part of a microscope that gathers light from the illuminator and focuses it into a light cone towards the specimen

Condenser

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10

Term for the hole of a microscope stage

Aperture

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11

Type of Microscope that is most commonly used and forms a dark image against a bright background

Bright field microscope

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12

Type of Microscope that permits detailed examination of internal structures of living organisms

Phase contrast microscope

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13

Type of Microscope that involves the excitation of fluorochromes by light to stain microorganisms

Fluorescent microscope

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14

Type of Microscope that forms a light image against a dark background

Dark field microscope

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15

Type of Microscope that uses electrons instead of light to visualize small objects

Electron microscope

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16

The ability of a microscope to visualize fine details and is used to distinguish two small objects at a specific distance apart

Resolution

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17

Magnification of Scanner objective

4x

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18

Magnification of Ocular

10x

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19

Magnification of LPO

10x

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20

Magnification of HPO

40x

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21

Magnification of OIO

100x

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22

Total Magnification of Scanner

40x

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Total Magnification of LPO

100x

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Total Magnification of HPO

400x

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Total Magnification of OIO

1000x

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26

Total Magnification used to view detailed fungal structures

1000x

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27

Substance or fluid used in OIO that has the same refractive index as glass

Cedar wood oil

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28

Term for when objectives that can be changed with minimal or no refocusing

Parfocal

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29

Most commonly used illumination in microscope

Tungsten Halogen lamp

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30

Term for the distance between the nosepiece opening to the top edge of the observation tubes wherein the eyepieces are inserted/mounted

Mechanical tube length

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31

Term for the distance between the objective lens and the surface of the specimen

Working distance (mm)

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32

Working distance of Scanner

27.8 mm

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33

Working distance of LPO

8.0 mm

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34

Working distance of HPO

0.6 mm

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35

Working distance of OIO

0.13 mm

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36

Disinfectant used for Microscope

70% Ethyl alcohol

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