MICR 2 LAB: Exercise 1 - Microscope

First person to experiment microorganisms "animacules"; Father of Microbiology

First person to experiment microorganisms "animacules"; Father of Microbiology

Antonie Van Leeuwenhoek

Inventor of compound microscope

Galileo Galilei

Coined the name "Microscope"

Giovanni Faber

Published Micrographia; Cell theory

Robert Hooke

Coined the term "Mycology"

Miles Joseph Berkley (1836)

Most common method used for detection and characterization of microorganisms

Microscopy

Equipment used in Microscopy

Microscope

Part of a microscope that controls the amount of light reaching the specimen

Iris Diaphragm

Part of a microscope that gathers light from the illuminator and focuses it into a light cone towards the specimen

Condenser

Term for the hole of a microscope stage

Aperture

Type of Microscope that is most commonly used and forms a dark image against a bright background

Bright field microscope

Type of Microscope that permits detailed examination of internal structures of living organisms

Phase contrast microscope

Type of Microscope that involves the excitation of fluorochromes by light to stain microorganisms

Fluorescent microscope

Type of Microscope that forms a light image against a dark background

Dark field microscope

Type of Microscope that uses electrons instead of light to visualize small objects

Electron microscope

The ability of a microscope to visualize fine details and is used to distinguish two small objects at a specific distance apart

Resolution

Magnification of Scanner objective

4x

Magnification of Ocular

10x

Magnification of LPO

10x

Magnification of HPO

40x

Magnification of OIO

100x

Total Magnification of Scanner

40x

Total Magnification of LPO

100x

Total Magnification of HPO

400x

Total Magnification of OIO

1000x

Total Magnification used to view detailed fungal structures

1000x

Substance or fluid used in OIO that has the same refractive index as glass

Cedar wood oil

Term for when objectives that can be changed with minimal or no refocusing

Parfocal

Most commonly used illumination in microscope

Tungsten Halogen lamp

Term for the distance between the nosepiece opening to the top edge of the observation tubes wherein the eyepieces are inserted/mounted

Mechanical tube length

Term for the distance between the objective lens and the surface of the specimen

Working distance (mm)

Working distance of Scanner

27.8 mm

Working distance of LPO

8.0 mm

Working distance of HPO

0.6 mm

Working distance of OIO

0.13 mm

Disinfectant used for Microscope

70% Ethyl alcohol