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Genetic Engineering Step 1 Study Guide
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1
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________ is negatively charged and it will move to the positively charged side.
DNA
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________- Used in PCR to take DNA through temperatures best for replication (95°, 50°, 72°)
Thermal cycler
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________- transfers DNA from gel onto membrane with probes.
Southern blotting
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________, found at top of test tube.
Supernatant suspends DNA
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The ________ makes a complementary strand using free nucleotides at 72°.
polymerase enzyme
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________ to carry genetic information with (often a plasmid)
Vectors Vessel
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________ and salts are added to clump debris.
Proteases
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________- degrades peptidoglycan in cell walls.
Lysosome
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________- single- strands of DNA or RNA that bind to a complementary sequence that have a biological marker attached.
Probes
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Precipitant- ________, found at bottom of test tube.
molecular debris
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________ uses primers which target specific sequences in DNA.
PCR
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________- non- coding sequences of DNA that can not leave the nucleus.
Introns
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________- Process where probe binds to complementary sequence.
Hybridization
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________- used to replicate specific sequence of DNA many times.
Polymerase chain reaction
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________ is added degrade peptidoglycan in cell walls.
Lysozyme
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________ are easy to cut and paste back together.
Plasmids
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________- Separates DNA fragments based on size.
Gel Electrophoresis
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________- manipulation of genetic information.
Genetic engineering
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________ that leave the nucleus.
Exons coding sequences
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________ can be extracted with alcohol.
DNA
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________ are added to lyse (explode) the cell.
Detergents
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________- Stands for Polymerase Chain Reaction, used to copy DNA many times.
PCR
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________ have short sequences of DNA.
Plasmids
24
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Stands for Polymerase Chain Reaction, used to copy DNA many times
PCR
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Separates DNA fragments based on size
Gel Electrophoresis
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