Genetic Engineering Step 1 Study Guide

________ is negatively charged and it will move to the positively charged side.

________ is negatively charged and it will move to the positively charged side.

DNA

________- Used in PCR to take DNA through temperatures best for replication (95°, 50°, 72°)

Thermal cycler

________- transfers DNA from gel onto membrane with probes.

Southern blotting

________, found at top of test tube.

Supernatant suspends DNA

The ________ makes a complementary strand using free nucleotides at 72°.

polymerase enzyme

________ to carry genetic information with (often a plasmid)

Vectors Vessel

________ and salts are added to clump debris.

Proteases

________- degrades peptidoglycan in cell walls.

Lysosome

________- single- strands of DNA or RNA that bind to a complementary sequence that have a biological marker attached.

Probes

Precipitant- ________, found at bottom of test tube.

molecular debris

________ uses primers which target specific sequences in DNA.

PCR

________- non- coding sequences of DNA that can not leave the nucleus.

Introns

________- Process where probe binds to complementary sequence.

Hybridization

________- used to replicate specific sequence of DNA many times.

Polymerase chain reaction

________ is added degrade peptidoglycan in cell walls.

Lysozyme

________ are easy to cut and paste back together.

Plasmids

________- Separates DNA fragments based on size.

Gel Electrophoresis

________- manipulation of genetic information.

Genetic engineering

________ that leave the nucleus.

Exons coding sequences

________ can be extracted with alcohol.

DNA

________ are added to lyse (explode) the cell.

Detergents

________- Stands for Polymerase Chain Reaction, used to copy DNA many times.

PCR

________ have short sequences of DNA.

Plasmids

Stands for Polymerase Chain Reaction, used to copy DNA many times

PCR

Separates DNA fragments based on size

Gel Electrophoresis