Studied by 0 peoplestable acid
Red /pink after methyl red: pos for ________ production.
addition of iodine
Clearing after ________: pos for amylase and starch hydrolysis.
Solid medium
________: neg for gelatinase.
Liquid medium
________: pos for gelatinase.
Thick strong growth
gram neg organism
No growth
no gram neg organism
Colorful (pink, black, purple, metallic green) growth
lactose fermentation
No colorful growht
no lactose fermentation
Opaque growth
gram pos
Transparent/Weak/No growth
not gram pos
Aerobic tube yellow & anaerobic tube green
pos for glucose oxidation only
Aerobic tube & anaerobic tube yellow
pos for glucose oxidation & fermentation
Aerobic tube green & anaerobic tube green
neg for glucose oxidation and fermentation
Clearing after addition of iodine
pos for amylase and starch hydrolysis
No clearing after addition of iodine
neg for amylase and starch hydrolysis
Yellow color
pos for fermentation
Red/Pink color
neg for fermentation and pos for pepone
Bubbles in Durham tube
gas production
No bubbles in Durham tube
no gas production
True fermentation
acid and gas production
Fermentation
acid production
No fermentation
no acid or gas production
Liquid medium
pos for gelatinase
Solid medium
neg for gelatinase
Fuchsia
pos for urease
Orange/yellow/no color
neg for urease
Black color
pos for H2S
No color change
neg for H2S
purple
pos for ornithine decarboxylase
yellow
neg for ornithine decarboxylase
red after kovacs reagent
pos for indole
no colar change after kovacs reagent
neg for indole
Forrest green color
pos
No color change
neg
Red/pink color after adding reagents A & B
pos (reduced to nitrate)
No color change after A, B, & Zinc
pos (reduced to nitrous oxide or nitrogen gas)
Red after A, B, & Zinc
neg (nitrate never reduced)
strip turns purple
pos
no color change
neg
Bubble
pos
No bubbles
neg
red/purple after kovacs
pos for indole and tryptophanase
no color change
neg for indole and tryptophanse
Red/pink after methyl red
pos for stable acid production
Yellow/orange after methyl red
neg for stable acid production
Red
post for acetoin production
gray/brown
neg for acetoin production
Blue
pos
Green
neg
What does it mean when strip does not change color in oxidase tset?
organism does not have cytochrome c
organism does not have cytochrome c
strict aerobe
Is catalase test selective or differential?
differential
What is catalase test differential for?
production of catalase
What does catalase do?
breaks down hydrogen peroxide into water and oxygen gas
What is a byproduct of aerobic respiration?
hydrogen peroxide
What does bubble mean in catalase test?
catalase is present
What does no bubbles mean in catalase test?
no catalase is present
What are IMViC tests used for?
identifying enterics
What are characterisitics of enterics?
faculative anaerobes, gram negative, non-endospore forming, bacilli, live in large intestine
All four of the IMViC tests are selective or differential?
differential
What does the indole test for?
production of endoenzyme tryptophanase and production of indole
What does it mean if there is a red/purple color after kovac's reagents in indole test?
organisms has indole and tryptophanase
What does it mean if there is no color change after kovac's reagent in indole test?
organism does not have indole or tryptophanse
What does methyl red test for?
production of stable acid from glucose
What does a red/pink color mean after adding methyl red in methyl red test?
organism is positive for stable acid production
What does it mean if there is a yellow/orange color after adding methyl red in methyl red test?
organism is negative for stable acid production
What does voges-proskauer test for?
production of acetoin (unstable acid production)
What is does in VP test after incubation?
VP 1 and VP 2 reagents added and cap is left off for ~30 min
What does a red color change mean in VP test?
organism does acetoin production
What does a gray/brown color change mean in VP test?
organism does not do acetoin production
What does citrate utilization test for?
if organism can use citrate as sole carbon source and produce citrase
What is pH indicator of citrate utilization test?
bromthymol blue
What does blue color change mean in citrate utilization test?
organism does use citrate and produces citrase
What does green color change mean in citrate utilization test?
organism does not use citrate or produce citrase
Note
Flashcard