Topic 2: Cultivation of Bacteria

Bacteria must be grown on ___ in order to characterize and identify it.

culture media

Bacteria require?

sources of energy, carbon, nitrogen, minerals, and growth factors

Most bacteria are ___ meaning they require organic compounds for carbon and energy sources.

chemoheterotrophs

Bacteria exhibit a wide range of nutritional requirements, therefore the addition of ___ like milk, serum, blood and tomato juice is need.

enriching nutrients

What are growth factors?

organic compounds such as vitamins or amino acids that are incorporated into a cell without alteration

The first bacterial cultures were grown in what type of media?

broth/liquid like infusions and blood

Who attempted to culture bacteria on potato slices when they realized the need for solid media?

Robert Koch

When Robert Koch realized that some bacteria wouldn't grow on potato slices, he ___.

added gelatin to liquid media

How come the addition of gelatin to liquid media didn't work?

gelatin liqueifed under incubation conditions

Who suggested the use of agar as solidfying agent?

Angelina Hess

What is the most commonly used solifying agent in culture media?

agar

Why is aseptic technique used?

to exclude contaminants; prevention of unwanted microorganisms in laboratory and medical procedures