Topic 2: Cultivation of Bacteria

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Bacteria must be grown on ___ in order to characterize and identify it.

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1

Bacteria must be grown on ___ in order to characterize and identify it.

culture media

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2

Bacteria require?

sources of energy, carbon, nitrogen, minerals, and growth factors

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3

Most bacteria are ___ meaning they require organic compounds for carbon and energy sources.

chemoheterotrophs

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4

Bacteria exhibit a wide range of nutritional requirements, therefore the addition of ___ like milk, serum, blood and tomato juice is need.

enriching nutrients

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5

What are growth factors?

organic compounds such as vitamins or amino acids that are incorporated into a cell without alteration

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6

The first bacterial cultures were grown in what type of media?

broth/liquid like infusions and blood

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7

Who attempted to culture bacteria on potato slices when they realized the need for solid media?

Robert Koch

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8

When Robert Koch realized that some bacteria wouldn't grow on potato slices, he ___.

added gelatin to liquid media

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9

How come the addition of gelatin to liquid media didn't work?

gelatin liqueifed under incubation conditions

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10

Who suggested the use of agar as solidfying agent?

Angelina Hess

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11

What is the most commonly used solifying agent in culture media?

agar

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12

Why is aseptic technique used?

to exclude contaminants; prevention of unwanted microorganisms in laboratory and medical procedures

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