DNA Replication Components and Functions

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9 Terms

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Ligase

seals the new strand by repairing the remaining nicks in the DNA

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DNA Polymerase III

attaches to the primer and adds nucleotides (the main polymerase in E. coli DNA replication)

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DNA Polymerase I

removes the RNA primer and replaces it with DNA

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Phosphodiester Bond

bonds joining nucleotides

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Single Stranded Binding Protein

binds to the strands of DNA to keep them separated

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Gyrase

relieves tension due to supercoils caused by strand separation

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Helicase

unwinds the DNA double helix

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Primase

lays down the RNA primer since DNA polymerase requires free 3' OH

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Primer

small piece of RNA needed for DNA polymerase to begin replication