DNA Sequencing, Electropherogram, Genomics, & Bioinformatics - 16B&C

What does DNA Sequencing mean?

A sequence of arrangement of nucleotide bases.

What is dideoxy chain termination.

using a special nucleotide called ddNTP’s to terminate a sequence

What are the ingredients of the dideoxy chain termination reaction?

• 16s rDNA copies

• one primer

• dNTPS

• ddNTPS

• Taq Polymerase

What 2 things are unique about ddNTPS?

• they only have one OH group and it is at the 2 prime carbon, so there is no OH group at the 3 prime carbon

• they have fluorescent labels color coded to the different bases

What is the product of dideoxy chain termination reactions?

millions of shortened copies of DNA ending with a ddNTP

What two things must be done to the fragments of dideoxy chain termination?

1. Ran through gel electrophoresis

2. Generate and electropherogram

What is gel electrophoresis?

arranging nucleotides based on size to determine the order of bases

What is an electropherogram?

A process where lasers are used to excite fluorescent labels and cameras capture the color patterns generating a visual record of the sequence

What is a contiguous sequence?

A sequence with no duplicates or overlapping bases, constructed using the 1530 Reverse primer

What does NCBI stand for?

Nation center for biotechnology information

What does BLAST stand for?

Basic Local Alignment Search Tool

What is genomics?

The study of multiple genomes as a functional unit?

What is Bioinformatics?

Computer and computational skills are used with an emphasis on statistical and machine learning techniques in biology.