Lab 9- Determination of Ketoses Using Seliwanoff’s Reaction

Seliwanoff's test

Timed color reaction for ketohexoses detection.

Ketohexoses

Sugars with a ketone group, e.g., fructose.

Aldoses

Sugars with an aldehyde group, e.g., glucose.

Colorimetric determination

Measurement of color intensity to quantify substances.

Dehydration reaction

Loss of water to form furfurals from sugars.

Resorcinol

Chemical used to form red complexes in reaction.

Hydrochloric acid

Concentrated acid used for sugar dehydration.

Absorbance measurement

Quantitative assessment of light absorption by samples.

Standard curve

Graph plotting absorbance against known concentrations.

Fructose concentration

Amount of fructose in a sample, measured in g/L.

Test samples

Substances tested for sugar content in experiments.

Water bath

Controlled temperature environment for reaction incubation.

False-positive result

Incorrect indication of sugar presence due to interference.

Cherry red-complex

Color produced by glucose under prolonged boiling.

Interference

Compounds affecting test results, leading to inaccuracies.

Seliwanoff's reagent

Solution of resorcinol in hydrochloric acid for testing.

Dilution

Process of reducing concentration of a solution.

Spectrophotometer

Instrument measuring light absorbance in samples.

Unknown fructose solution

Sample with unknown fructose concentration to analyze.

Clinical chemistry

Field applying chemical analysis for medical diagnostics.

Prolonged boiling

Extended heating that can alter sugar structures.

Tomato sample

Test subject for measuring fructose concentration.

Standard samples

Known concentrations used to create a calibration curve.