Water Analysis and Media Preparation

MAC

________ inhibits the growth of gram positive organisms.

TCBS

_______ is a differential media for Salmonella spp

37 degrees Celsius for 24-28 hours

In the confirmatory test of the multiple tube fermentation technique, BGLB is incubated at ______________________ before results are determined.

TRYPTOSE LAURYL SULFATE BROTH

In the multile tube fermentation technique, ______________ is used in the presumptive test.

LIA

__________ is a medium used to determine decarboxylation, motility, and indole production.

ENRICHED/DIFFERENTIAL

BAP is an ______________ culture medium

Thiosulfate-Citrate-Bile Salts-Sucrose Agar

acronym: TCBS

Alkaline Peptone Water

acronym: APW

Sulfide-Indole-Motility Medium

acronym: SIM

Brain Heart Infusion Broth

acronym: BHIB

Triple Sugar Iron Agar

acronym: TSI

A selective inhibitory medium used to isolate Vibrio spp.

TCBS

Enrichment medium fore Vibrio spp

APW

Differential medium to detect hydrogen sulfide production, indole production, and motility.

SIM

A nutrient-rich medium for growth of Staphylococci and fastidious organisms

BHIB

Differential medium for identifying gram-negative enteric bacteria based on sugar fermentation and production of hydrogen sulfide.

TSI

a. Presumptive test

b. Confirmatory test

c. Completed test

General steps included in the multiple tube fermentation technique used in the bacteriologic analysis of water:

a. Glucose (0.1%)

b. Lactose (1.0%)

c. Sucrose (1.0%)

Sugars (with their concentration) present in TSI:

a. Quality control of cultured media

b. sterility test

c. performance test

d. interval control procedure

Quality assurance procedure for prepared culture media: