Water Analysis and Media Preparation

MAC inhibits the growth of gram positive organisms.

SSA is a differential media for Salmonella spp.

In the confirmatory test of the multiple tube fermentation technique, BGLB is Incubated at 37 degrees Celsius fot 24-28 hours before results are determined.

In the multiple tube fermentation technique, TRYPTOSE LAURYL SULFATE BROTH is used in the presumptive test.

LIA is a medium used to determine decarboxylation, motility, and indole production.

6. BAP is an ENRICHED/DIFFERENTIAL culture medium.

Acronyms and Their Laboratory Use

TCBS (Thiosulfate-Citrate-Bile Salts-Sucrose Agar): A selective inhibitory medium used to isolate Vibrio spp.

APW (Alkaline Peptone Water): Enrichment medium fore Vibrio spp.

SIM (Sulfide-Indole-Motility Medium): Differential medium to detect hydrogen sulfide production, indole production, and motility.

BHIB (Brain Heart Infusion Broth): A nutrient-rich medium for growth of Staphylococci and fastidious organisms.

TSI (Triple Sugar Iron Agar): Differential medium for identifying gram-negative enteric bacteria based on sugar fermentation and production of hydrogen sulfide.

General steps included in the multiple tube fermentation technique used in the bacteriologic analysis of water:

a. Presumptive test

b. Confirmatory test

c. Completed test

Sugars (with their concentration) present in TSI:

a. Glucose (0.1%)

b. Lactose (1.0%)

c. Sucrose (1.0%)

Quality assurance procedure for prepared culture media:

a. Quality control of cultured media

b. sterility test

c. performance test

d. interval control procedure