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cell fractionation
the disruption of a cell and separation of its parts by centrifugation at successively higher speeds (lower speeds=larger components, higher speeds=smaller)
eukaryote
has membrane bound organelles, DNA is in the nucleus, consists of plants, animals, fungi, and protists
prokaryote
does NOT have membrane bound organelles, DNA resides in a space called the nucleoid, consists of bacteria and archaea
cell/plasma membrane
a selective barrier around ALL CELLS that is SEMIPERMEABLE and regulates its chemical composition, made up of a phospholipid bilayer that has hydrophilic heads facing outwards and hydrophobic tails facing inward
cytosol
the semifluid, gel-like substance inside cells where organelles are suspended
nucleoid
the area in prokaryotic cells where the DNA is generally located
cytoplasm
the contents of the cell bound by the cell/plasma membrane, in eukaryotes: the portion exclusive of the nucleus
organelles
different environments in the cell that each carry out specific metabolic functions MEMBRANE BOUND
nucleus
organelle that contains genetic material (DNA) in the form of chromosomes, made out of chromatin
nuclear envelope
the double membrane that surrounds the nucleus, dotted with pores that regulate traffic with the cytoplasm, the outer membrane is continuous with the endoplasmic reticulum (ER)
nuclear lamina
a net like array of protein filaments that lines the nuclear envelope and helps maintain its shape
chromosomes
structures that carry genetic information
chromatin
the complex of DNA and proteins that make up chromosomes
46
how many chromosomes does a human have in the nucleus
nucleolus
the site of rRNA synthesis (ribosomal RNA), also synthesizes ribosome parts, may play a role in cell division and the life of a cell
ribosomes
cellular components that carry out protein synthesis
free ribosomes
ribosomes suspended in cytosol- mainly produce proteins that function WITHIN THE CELL/CYTOSOL
bound ribosomes
ribosomes on the rough ER/nuclear envelope- mainly make proteins inserted into other membranes, or for secretion from the cell (export)
endomembrane system
system that contains both ERs, golgi, lysosomes, vesicles/vacuoles, and the plasma membrane
vesicle
sacs made out of membrane inside or outside the cell, can be used to transport proteins/other stuff
Smooth ER
organelle that synthesizes lipids (oils, steroids, new membrane phospholipids), metabolizes carbohydrates, detoxification of drugs and poisons, and stores calcium ions
Rough ER
makes secretory proteins from its bound ribosomes, also a membrane factory- produce membrane phospholipids
glycoproteins
a protein with one or more covalently attached carbohydrates
golgi apparatus (golgi body)
a stack of flat, membranous sacs that modifies, stores, and routes proteins (products of the ER), and synthesizes some other products like noncellular carbs
cis face
receiving side of the golgi apparatus
trans face
shipping side of the golgi apparatus
lysosome
a sac of hydrolytic enzymes that digests macromolecules; can be used to break down old cell material or even fight viruses and bacteria
phagocytosis
process of large particles or small organisms being taken up by a cell and then digested
vacuoles
stores extra molecules cell might need- water, lipids, carbs, etc. the large central one of these contains inorganic ions such as potassium and chloride
mitochondria
serves as the site of cellular respiration; uses oxygen to break down organic molecules then synthesize ATP (FOUND IN BOTH ANIMAL AND PLANT)
chloroplasts
sites of photosynthesis in plants cells, absorbs sunlight and uses carbon dioxide and water to make sugars for energy
endosymbiont theory
theory that mitochondria and chloroplasts were actually engulfed by the cell as prokaryotes and then evolved to be with its host cell
cristae
folds of the inner mitochondria membrane, increases the surface area in order to increase efficiency/reaction number and rate of the organelle
mitochondrial matrix
inclosed by the inner membrane of the mitochondria, where cellular respiration occurs
thylakoid
flattened sac in a chloroplast that carries out photosynthesis (light→chemical energy)
grana
stacks of thylakoids
stroma
fluid outside the thylakoids
intermembrane space
space between the inner and outer membranes of a chloroplast
plastids
amyloplast, chloroplast, chromoplast, found in photosynthetic eukaryotes
peroxisomes
removes hydrogen atoms from various substances and transfers them to oxygen, producing hydrogen peroxide, can be used to break down fatty acids for cellular respiration, or to detoxify alcohol. After converting to hydrogen peroxide, they also have an enzyme to convert that to water
cytoskeleton
a network of fibers extending throughout the cell that gives it its structure- consists of microtubules, microfilaments, and intermediate filaments
microtubules
thickest of three structural elements, they shape and support the cell as well as serve as tracks for which organelles equipped with motor proteins can move, also make up the centrosome/centrioles
centrosome
functions as a microtubule organizing center and is important in cell division, contains two centrioles that are also important in cell division
flagella
a long tail some cells use for motility, made of microtubules
cilia
moves fluid past the cell, kind of like little hairs, made of microtubules
microfilaments
thin solid rods that pull and bear tension to keep structure in place, also play a role in cell motility
intermediate filaments
reinforces the shape of the cell and fixing the position of certain organelles
cell wall
a protective layer external to the cell wall in plants, prokaryotes, fungi and some protists; made of cellulose and peptidoglycan
middle lamella
layer of adhesive extracellular material, found between primary walls of adjacent young cells
extracellular matrix (ECM)
meshwork surrounding animal cells, consisting of glycoproteins, polysaccharides, and proteoglycans, secreted by cells
plasmodesmata
an open channel through cell walls of adjacent plant cells, connecting the cytoplasm and allowing stuff to flow through
desmosomes
function like rivets, creating sturdy sheets of cells