IACUC

USDA, PHS, OLAW

organizations that regulate animal research

AWA

the act that gives the USDA broad authority to regulate animal research

when two or more species are appropriate for a research or educational project, IACUC may request that the ____ ____ one is used

what are the three Rs of minimizing animal pain and use

neonates are resistant to

true

true or false: if a surgical procedure would be expected to cause pain in a human, it should be assumed to cause pain in an animal

analgesic, anesthetic

_____ relieve pain without loss of feeling, whereas _____ relieve most feeling and may be accompanied by loss of consciousness

false

true or false: treatments under an organization’s occupation health program are required

aseptic

sterile or _____ technique refers to a series of practices intended to prevent the contamination of the surgical site by microbes during surgery

rodents, rabbits

_____ and ______ should not be fasted prior to surgery: they are unable to vomit due to their esophageal physiology, such that there is no risk of aspiration pneumonia (breathing in vomit)

false

true or false: cost savings alone is an adequate justification for performing multiple major survival surgeries on the same animal

investigators must receive ____ _____ in order to contract with a commercial enterprise or other entity to produce custom antibodies

one, one

a general guideline for blood collection is that no more than __ percent of the body weight as blood should be collected at once, assuming that a milliliter of blood weights __ gram

two to four weeks

blood collections of no more than one percent of body weight can usually be performed every ______ without harm

false

true or false: blood collection from the heart (cardiocentesis) can be performed as a non-terminal procedure

wire mesh flooring

solid bottom caging with bedding is recommended for most laboratory rodents as opposed to ___ ____ ____

minor

a change in laboratory location is considered a ____ (minor/major) protocol change

false

true or false: the Veterinary Verification and Consultation (VVC) is a mandatory method of IACUC review applying to certain types of protocol changes

surgical gloves, masks, sterile instruments, aseptic techniques

4 basic requirements for aseptic technique according to animal welfare regulations

ethylene oxide

type of gas used for chemical sterilization which is non=corrosive and safe for many polyethylene/plastic materials

false

true or false: ethylene oxide can be used for liquids/when wrap or packaging is gas impervious

true

true or false: animals should be prepared for surgery in a separate area from surgical operating room to maintain cleanliness