BIO lab 9

What determines which part of the DNA template will be amplified during PCR

Primers

Which electrode does DNA move toward during electrophoresis? What part of the DNA is responsible for its movement towards

- charged DNA moves towards the positive electrode

The phosphate groups along the backbone of DNA will migrate

Who committed the crime

C

Why must we use genetics to tell some species apart

Some

Comparison of whole genome sequences provides a highly detailed view of how organisms are related to each other at the genetic level. How are genomes compared and what can these findings tell us about how the overall structure of genes and genomes have evolved?

What about dwarf lemurs that makes them more difficult to study compared to some other species of lemur

They hibernate for the significant part of the year

Why is the cytb gene sequence often used for identifying species

it shows limited variability within and much greater variation between species.

PCR RFLP:

Starts by using OCR and make copies of the lemur mitochondrial genome.

2 mutations in the cytp gene in crossly

these mutations allow enzyme bl 2 to cut the cytb pcr product from crossly but not from other species. If the enzyme does not cut the fragment the DNA is from a different species

DNA ladder

we load this so we can know the diff sizes of DNA and compare it to the unknown DNA sample and identify the sizes

There are more species of dwarf lemur recognized today than there were 20 years ago. How were these species identified and why weren't they recognized previously