________ in the tRNA anti- codon: 3rd base is less constrained and pairs less specifically.
2
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Release factors
________ (RFs) bind to stop codon and assist the ribosome in terminating translation.
3
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Eukaryote AUG codon
________ is embedded in a short initiation sequence called the Kozak sequence.
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5
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6
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7
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8
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9
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10
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11
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12
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13
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14
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15
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16
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17
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18
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19
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20
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21
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22
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23
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24
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25
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26
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Code is non-overlapping
each nucleotide is part of only one codon and is read only once
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Code is almost universal
most codons have the same meaning in different organisms
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Code is degenerate
18 of 20 amino acids are coded by more than one codon
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Code has start and stop signals
AUG codes for Met and is the usual start signal
30
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Wobble occurs in the tRNA anti-codon
3rd base is less constrained and pairs less specifically
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P site
peptidyl site
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A site
aminoacyl site
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E site
exit site
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Initiation
a functionally competent ribosome is assembled in the correct place on the mRNA and ready to start protein synthesis
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Elongation
tRNA brings the correct amino acid to the ribosome, it is joined to the nascent polypeptide chain and the entire assembly moves to the next triplet along the mRNA
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Termination
a stop codon is reached and the entire assembly dissociates to release the newly-synthesized polypeptide
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Initiation factor (IF
4F) binds to the 5 cap on the mature mRNA
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IF 1
associates with 30S subunit in the A site 🡪 preventing a tRNA from entering
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IF 3
allows for 30S subunit to bind to specific site of mRNA, checks for accuracy of binding of first aa tRNA
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IF 2-GTP
binds to 30S P site upon which fmet-tRNA binds to IF2 and IF2 moves it to P site
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Peptidyl transferase (catalytic RNA molecule
ribozyme) forms a peptide bond between the free amino acid in the P site and aminoacyl-tRNA in the A site