Recombinant DNA Technology
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4 Terms
What is Recombinant DNA Technology?
Transfer of DNA fragments from one organism/species to another
Why can transferred DNA be translated within cells of recipient organisms?
Genetic code is universal
Transcription and translation mechanisms are universal
Methods of producing DNA fragments?
Reverse transcriptase for conversion of mRNA to complementary DNA
Restriction enzymes to cut fragments of desired gene
Gene machine
Process of using reverse transcriptase for conversion of mRNA to complementary DNA?
Isolate mRNA from cells that express the gene for the desired protein
Mix mRNA with DNA nucleotides and reverse transcriptase to produce single strand of cDNA
DNA polymerase forms a second strand
= double stranded DNA copy of desired gene with NO introns
Note 
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