Studied by 6 people
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1st Postulate
Microbe should be in diseased person, but not healthy person
2nd Posulate
Microbe can be cultured from diseased person
3rd Posulate
Microbe from culture will cause disease when put into healthy person
4th Postulate
Microbe can be re-isolated and it will be the same as before
Cell culture
process of growing cells in a laboratory
Agar
solid media
Broth cultures
liquid media (better acess to nutrients)
Simple media
has only a few nutrients (no special requiremtns)
Selective media
has additives that allow only certain cells to grow
Enriched media
additional growth factors (blood agar)
Differential media
allows multiple microbes to grow but form individual colonies
Koch鈥檚 postulates
four criteria that are used to to identify the causative agent of a particular disease
Autoclave
sterilizes things using heat, pressure, & steam
Lypholization
Where the water is removed from the thing (ex. dried bacteria)
Primary cells
Cells taken directly from animals
Cell lines
Primary cells that are made imortal
Contact inhibition
Where eukaryotic cells stop growing when they touch each other
Fermenters
where large-scale cell cultures are grown in suspension broth cultures
Microbiological tools
inoculating loops & needles
Serial dilution
diluting petri dish by a known factor (used to count numer of cells)
Quadrant streak
Inoculating 4 quadrants on petri dish to isolate colonies
T-streak
Same as quadrant streak but with only 3 quadrant
Colony forming units
number of cells
Continuous streak
One zig-zag across the dish
Sterilization
to destroy all microbial life + endospores
Disinfection
to destroy microbal life but NOT endospores
Antisepsis
use of liquid antimicrobial chemical on skin or living tissue to destroy microorganisms
Decontamination
process to make something safe to handle
Cleaning
use of water and detergent to remove materials & reduce number of microorganisms
Pluripotent stem cells
Can differentiate into any cell in the human body
Adult stem cells
In tissue/organ and can specialize only to cells in that tissue/organ
Liquid Media
gives cells better access to nutrients
Factors that influence cell growth
Temperature, pH, oxygen and availiable nutrients
Note 
Flashcard (100)