(Problem Set 1) Techniques/ Microscopy

This is about section one of Neuro 302, all about Microscopy and techniques, no images used, only multi choice

What allows transgenic animals to pass on their transgene to their offspring?

The transgene is inserted into the male pronucleus.

CLARITY processing

Removal of lipids

Which microscopy technique is most affected by photobleaching and phototoxicity?

Confocal fluorescence microscopy.

A form of microscopy that permits the visualization of the smallest organelles.

Electron microscopy

A technique used to measure radioactive probe location and density in brain slices.

Autoradiography

A technique that can be utilized to measure horseradish peroxidase precipitate.

Light microscopy

A cell body stain that highlights RNA in cells.

Cresyl violet

A non-cell permeable nuclear stain used for identifying dead cells.

PI stain

A stain that allows for whole neuronal staining.

Golgi-Cox stain

A cell-permeable nuclear stain used for visualizing nuclei.

DAPI stain

A type of microscopy that provides images in a single tissue plane.

Confocal microscopy

What technique is used to measure Huntingtin protein levels in brain tissue?

Immunohistochemistry.

Which of the following statements is correct about secondary antibodies?

Several of them bind to the same primary antibody

What is INCORRECT about Golgi-Cox staining?

It does not utilize heavy metals such as silver.

Brainbow technique is creased using viral vector mediated gene transfer

False

How does confocal microscopy improve resolution?

By restricting the path of light using pinpoint disks.