(Problem Set 1) Techniques/ Microscopy

Some transgenic animals can pass on their transgene to their offspring. This is because...The transgene is inserted into the male pronucleus.

What permits increased resolution of imaging following CLARITY-processed brain compared to an untreated brain? Removal of lipids

Which microscopic technique is most affected by photobleaching and phototoxicity?

confocal fluorescence microscopy

This form of microscopy permits the visualization of the smallest organelles. electron microscopy

What technique can be used to measure radioactive probe location and density in brain slices? autoradiography

Confocal microscopy: most likely to elicit photobleaching

Epifluorescence microscopy: most likely to suffer from high autofluorescence

Electron microscopy: the most expensive

Light microscopy: can be utilized to measure horseradish peroxidate precipitate

Cresyl violet: Cell body stain (RNA)

PI stain: Non-cell pearmeable nuclear stain

Golgi-Cox stain: Whole neuronal stain

DAPI stain: Cell-permeable nuclear stain

Which type of microscopy provides images in a single tissue plane? confocal microscopy

You wish to measure the levels of Huntingtin protein in brain tissue obtained from a Huntington's disease patient.

Immunohistochemistry

Which of the following statements is correct about secondary antibodies?

Several of them bind to the same primary antibody

Which statements are INCORRECT about Golgi-Cox staining

Utilizes heavy metals such as silver

Brainbow is creased using viral vector mediated gene transfer.

False

How does confocal microscopy improve the resolution of fluorescence microscopy?

By restricting the path of light using pinpoint disks