Eosin- Methylene Blue Agar

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Description and Tags

micro lab 2 specific agars

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6 Terms

1
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SELECTS for gram (-)

Suppresses growth for gram +

2
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Purpose of Eosin -Methylene Blue tests

To identify what sort of lactose fermenter a bacteria is

3
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What are the components of Eosin Methylene Blue Agar?

  1. gelatin- provides nitrogen and organic carbon

  2. lactose

  3. the dyes eosin Y and methylene blue

4
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Dark purple or black with a green sheen indicates more aggressive lactose fermenters

  • Salmonella

  • Shigella

  • Proteus

5
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Less aggressive lactose fermenters turn what color?

Pink to dark purple

  • Enterobacter

  • Klebsiella

  • E. Colie

6
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Lactose non- fermenters ( don’t care)

Take on their normal color or take on the color of the media (colorless colonies)

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