What are the sources of microbial crude protein?
dietary protein and recycled nitrogen from the rumen
How do you calculate dietary crude protein?
CP= Nitrogen x 6.25
What are the functions of proteins?
Building blocks of enzymes and hormones, immune system, transport in the body, and tissue/structural
What is in the general structure of an amino acid?
alpha-amino end, generic amino acid, carboxyl end
What are the 10 essential amino acids? (PVT TIM HALL)
Phenylalanine, Valine, Threonine, Tryptophan, Isoleucine, Methionine, Histidine, Arginine, Leucine, Lysine
What are non-essential amino acids?
alanine, arginine, asparagine, aspartic acid, cysteine, glutamic acid, glutamine, glycine, proline, serine, tyrosine
What is the primary structure of a protein?
linear sequence of amino acids joined by peptide linkages
What is the secondary structure of a protein?
shape of the protein due to hydrogen bonding between the carboxyl and amino groups
What is the tertiary structure of a protein?
folding and coiling of protein into globular 3D structure
What is the quaternary structure of a protein?
alignment of several tertiary structures to form a single protein
What is a simple protein?
proteins yielding only amino acids or their derivatives on hydrolysis
What is a fibrous protein?
protein that constitute about 30% of total protein in the animal body
What is a conjugated protein?
proteins in which simple proteins are combined with a nonprotein radical
What protein is considered to have the highest BV of natural sources?
egg protein
What are some deficiencies of proteins?
growth restriction, decreased feed efficiency, infertility, anorexia, fatty liver, decrease milk production, reduced synthesis of certain hormones and enzymes
Microbial crude protein has a high value of biological value. What is the BV?
66-87
What is metabolism?
sum of all biochemical processes that nutrients undergo to furnish energy and build new tissue
What is anabolism?
building up
What is catabolism?
breaking down molecules
Where does transcription take place?
nucleus
What is transcription?
synthesis of an RNA molecule from a DNA template
Where does translation occur?
cytoplasm
What is translation?
the decoding of an mRNA message into a protein
Catabolism is the breakdown of amino acids and occurs primarily in the liver.
When does the rate of catabolism increase?
when dietary proteins exceed supply requirements, composition of absorbed AA is unbalanced, gluconeogensis from AA is increased
Digestion of protein
hydrolysis to peptides and AA; absorption of peptides and free amino acids
Transport of amino acids to the liver
Amino acids are taken to the liver via the hepatic portal vein; Liver has first choice in removing amino acids for protein synthesis and oxidation
Metabolism of amino acids in the liver
If the liver has the appropriate enzymes, excess amino acids will be converted to glucose; Amino acids first are used to satisfy protein synthesis
release of essential amino acids
Used by other tissues for protein synthesis; Excess BCAAs transaminated in the muscle; their carbon skeletons are oxidized in the liver
What makes up a triglyceride?
glycerol and 3 fatty acids
What is a cis bond?
Hydrogen is on the same side of the double bond
What is a trans bond?
Hydrogen on the opposite side of the double bond
What does it mean to be saturated?
having no double bonds
What does it mean to be unsaturated?
contains at least one double bond
Fat has how much more energy than carbs?
2.25
What are plant sources of lipids?
oilseeds, soybeans, cottonseed, canola, grain, corn, milo, wheat
What are animal sources of lipids?
tallow
Fats
usually of animal origin, saturated, solid at room temperature
Oils
Usually of plant origin, unsaturated, and liquid at room temperature
Types of simple lipids are?
true fats and waxes
Types of compound lipids are?
phospholipids, glycolipids, lipoproteins
Types of derived lipids are?
sterols and fatty acids
What is super important in the membrane?
phospholipids
What are chylomicrions?
formed inside small intestine mucosal cells; majority of fat carried transported out of small intestine
What are VLDL?
contain high levels of TAG, transports lipids from liver to extra hepatic tissues, tissue cells secrete LPL which breaks down TAG to free fatty acids and glycerol
What are HDL?
produced in the liver and small intestines, will bind excess cholesterol and carry back to the liver for excretion
What are the EFA?
linoleic acid, linolenic acid, arachidonic acid
Prostaglandin helps in what?
reproduction
What is the precursor for steroid hormone production?
cholesterol
What are eicosanoids synthesized from?
arachidonic acid
What are the three classes of eicosanoids?
prostaglandins, thromboxanes, leukotrienes
Common name: lauric
Geneva name: dodecanoic
Common name: myristic
geneva name: tetradecaonic
Common name: palmitic
geneva name: hexadecaonic
Common name: stearic
geneva name: octadecanoic
What species does not store bile in the gallbladder?
Horses- their liver continously secret bile acids
What are a form of lipoproteins circulating in the blood?
chylomicrons
Carbon sources of fatty acids synthesis will vary based on diet and mode of digestion.
Mono-gastrics utilize what as major source?
glucose
Ruminants use what as a major source?
acetate
Fatty acids are built how many carbons at a time?
2
Increasing rate of fatty acid synthesis increases what?
energy provided by the diet (excess fat, glucose, AA)
Lipogenesis substrates
acetate, propionate, lactate, and glucose
Ruminants have evolved to effectively utilize what?
acetate
What is important in ruminants?
propionate
Most of the carbon from glucose enters fatty acid synthesis via glycolysis and the production of pyruvate.
Major sites of fatty acids synthesis are?
liver, adipose tissue, mammary glands
Catabolism of TAGs occur in all tissues that have fat storage.
What is lipolysis stimulated by?
stimulated by an energy deficit/stress
Increased NEFA concentrations means?
increased lipolysis
Majority of lipid digestion starts in the what of monogastrics?
stomach
Describe the emulsification of fats.
the breakdown of fat into smaller droplets which increase surface area so that pancreatic lipase can digest it into the glycerol backbone and fatty acids