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Transcription
Process that converts DNA Nucleic Acid language into RNA Nucleic Acid language.
RNA Structure
• Ribose sugar.
• Nitrogen bases: Uracil, Adenine, Cytosine, Guanine.
• Single stranded.
Template Strand
Transcribed DNA strand; the strand of DNA that is temporarily paired with the RNA.
Transcription Bubble
Where DNA unzips for transcription to begin.
RNA Polymerase
Transcribes genes into mRNA.
Promoter Region
Binding site before beginning of gene; for RNA Polymerase and transcription factors.
TATA Box Binding Site
A DNA sequence in eukaryotic promoters crucial in forming the transcription initiation complex.
Exons
The real gene; expressed/coding DNA
Introns
The junk genetic material; IN between sequence.
mRNA Splicing
Post-transcriptional processing; edits out introns.
Mature mRNA
The final product of mRNA splicing.
Spliceosome
Several snRNPs. Recognize splice site sequence. "Cut and paste" gene.
Alternative Splicing
Alternative mRNAs produced from same gene; different segments treated as exons.
Post-Transcriptional Processing
Need to protect mRNA on its trip from nucleus to cytoplasm; enzymes in cytoplasm attack mRNA.
mRNA Caps
5' GTP Cap and Poly-A Tail; longer tail, mRNA lasts longer: produces more protein.
Reading Frame
Reading mRNA nucleotides in the correct groupings. If a single base is added or lost, it throws off the entire reading frame.