Ch. 12 Notes Components required for replication

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8 Terms

1
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What is the function of the initiator protein in DNA replication?

Binds to the origin and separates DNA strands to initiate replication.

2
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What role does DNA helicase play during DNA replication?

Unwinds DNA at the replication fork.

3
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What do single-strand binding proteins do during DNA replication?

Attach to single-stranded DNA and prevent secondary structures from forming.

4
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What is the function of DNA gyrase during replication?

Moves ahead of the replication fork, making and resealing breaks in DNA to release torque from unwinding.

5
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What does DNA primase do in DNA replication?

Synthesizes a short RNA primer to provide a 3’-OH group for DNA polymerase.

6
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What is the role of DNA Polymerase III in DNA replication?

Elongates a new nucleotide strand from the 3’-OH group provided by the primer.

7
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What is the function of DNA Polymerase I in DNA replication?

Removes RNA primers and replaces them with DNA.

8
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What does DNA ligase do during DNA replication?

Joins Okazaki fragments by sealing nicks in the phosphate backbone of newly synthesized DNA.