MLSC 2131: Midterm II full set W4-W7
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The following are all obligate intracellular pathogens, EXCEPT for:
Treponema
3 multiple choice options
true or false: very difficult to culture organisms can only be grown on chocolate agar in CO2
false, chocolate agar is not enough to grow these organisms
3 multiple choice options
how do we diagnose infection with a pathogen that is impossible to grow?
serological or molecular methods
3 multiple choice options
when diagnosing an impossible to grow pathogen, which of the following would an antibody test be looking for?
IgG or IgM
3 multiple choice options
what do Chlamydiaceae, Ricketsiaceae and Coxiella have in common?
they all need human cells to survive
3 multiple choice options
which of the following is true of Chlamydia and Rickettsia?
they are very small - smaller than a human mitochondria
3 multiple choice options
which of the following organism families has two forms that make up its reproductive cycle?
Chlamydiaceae
3 multiple choice options
which of the following refers to a metabolically inactive, infectious form of a bacteria used for its extracellular survival?
Elementary Body
3 multiple choice options
which of the following refers to a metabolically active, noninfectious form of a bacteria used for replication?
Reticulate Body
3 multiple choice options
which of the following pathogen has some serotypes that cause the most common STI in Canada, can infect neonates, and is the major cause of infertility?
Chlamydia trachomatis
3 multiple choice options
which of the following can be cultured?
Chlamydia trachomatis
2 multiple choice options
what is the preferred method for the laboratory diagnosis of Chlamydia trachomatis?
PCR molecular
3 multiple choice options
How does Chlamydophila pneumoniae spread within the body?
once infected cell lyzes, infection spreads to nearby cells
3 multiple choice options
under what temperature should Chlamydophila pneumoniae be stored?
2-8 C
3 multiple choice options
which of the following is spread via vector transmission and causes Rocky Mountain Spotted fever?
R. rickettsii
3 multiple choice options
true or false: the recommended method to diagnose Rickettsiae is cell culture
false
1 multiple choice option
which of the following facilities is able to store and test Rickettsiae?
Level 3 lab
3 multiple choice options
which of the following would you use to collect a sample for Rickettsiae diagnosis?
Serum (SST)
3 multiple choice options
which of the following is a potential bioterrorism agent that causes Q fever?
Coxiella burnetti
3 multiple choice options
which of the following is used for laboratory diagnosis of Coxiella burnetti?
Indirect Immunofluorescence antibody (IFA) assay for IgG and IgM
3 multiple choice options
all of the following are spirochete genera that cause disease in humans, EXCEPT for:
Coxiella
3 multiple choice options
which spirochete genera is transmitted mucous membrane to mucous membrane?
Treponema
3 multiple choice options
which spirochete genera is transmitted via vector?
Borrelia
3 multiple choice options
which spirochete genera is transmitted via zoonoses?
Leptospira
3 multiple choice options
which of the following atypical bacteria causes syphilis?
Treponema pallidum
3 multiple choice options
which of the following would most likely be used for laboratory diagnosis of Treponema pallidum?
Direct fluorescent microscopy antigen testing
3 multiple choice options
which of the following organisms is known to exhibit 3 phases of disease including chancres, disseminated lesions, and tissue involvement?
Treponema pallidum
3 multiple choice options
which of the following is true of Treponema pallidum?
Primary phase of disease is marked by a chancre that may disappear within a few weeks
3 multiple choice options
which is the most infectious stage of Treponema pallidum?
Secondary phase
3 multiple choice options
which stage of Treponema pallidum infection is usually not considered transmissible?
Tertiary phase
2 multiple choice options
what is the preferred method for laboratory diagnosis of Treponema pallidum?
Direct detection from lesions or antibody testing
3 multiple choice options
true or false: a negative result for fluorescent antibody staining can rule out a syphilis infection
false
1 multiple choice option
a nontreponemal serologic test includes which of the following:
rapid plasma reagin tests
2 multiple choice options
which of the following conditions can cause a false positive in a rapid plasma reagin tests?
pregnancy
3 multiple choice options
which of the following bacteria is responsible for causing lyme disease?
Borrelia burgdorferi
3 multiple choice options
which of the following is true of Borrelia burgdorferi?
it is a spiral-shaped bacterium
3 multiple choice options
what are the growth requirements for Borrelia burgdorferi?
specialized media at 33 C for 6 weeks or more
3 multiple choice options
which of the following can be transmitted indirectly if you open your eyes in contaminated water?
Leptospira
3 multiple choice options
what is the preferred method for laboratory diagnosis of Leptospira?
Serologic testing for presence of IgM
3 multiple choice options
a serological test for Leptospira has come back positive for IgG, and negative for IgM, what does this mean?
the patient has had contact with the pathogen in the past and has developed immunity, but does not have a current infection
3 multiple choice options
which of the following is true of mycoplasma?
they do not have a cell wall
3 multiple choice options
which mycoplasma is an obligate aerobe?
M. pneumoniae
2 multiple choice options
which of the following is responsible for atypical pneumonia?
Mycoplasma pneumoniae
3 multiple choice options
what is the preferred method for laboratory diagnosis of Mycoplasma?
Molecular diagnostics
3 multiple choice options
Boxcar bacilli are indicative of which GPB?
Bacillus s.p
3 multiple choice options
Short bacilli are indicative of which GPB?
Listeria
3 multiple choice options
Branching/beading is associated with which of these GPB?
Nocardia
3 multiple choice options
V forms and palisades are associated with which GBP?
Corynebacterium
3 multiple choice options
A ground glass morphology could be indicative of which organism?
Bacillus
3 multiple choice options
An organism has the following characteristics:
1. Beta hemolytic double size of colony
2. Large, flat
3. GPB
4. Boxcar shape
5. Anaerobic growth, but aerotolerant
6. Reverse CAMP +
which ID would you suspect?
Clostridium perfringens
3 multiple choice options
An organism has the following characteristics:
1. Large, irregular, ground glass
3. GPB
4. Aerobic growth
5. Catalase +
6. Motile
which ID would you suspect?
Bacillus
3 multiple choice options
An organism has the following characteristics:
1. Alpha hemolytic, opaque, tiny
3. GPB
4. Facultative anaerobe
5. Catalase -
6. Non motile
which ID would you suspect?
Lactobacilli
3 multiple choice options
Which organism is described as having a "breadcrumb" appearance?
Nocardia
3 multiple choice options
An organism has the following characteristics:
1. Beta hemolytic, translucent, grey
3. Short GPB
4. Catalase +
6. Tumbling motility
which tests would you choose to complete ID?
Bile esculin > vitek GP
3 multiple choice options
which GPB is alpha hemolytic?
Lactobacilli
3 multiple choice options
all of the following GPB are Catalase + EXCEPT for:
Lactobacilli
3 multiple choice options
Bacillus spp is considered normal flora when found in:
skin, wound swabs
3 multiple choice options
what is the typical mode of transmission for Bacillus anthrasis?
contact with infected animals
3 multiple choice options
what is the typical source of infections with Listeria?
food
3 multiple choice options
which small GPB is motile at lower temperatures?
Listeria
3 multiple choice options
which of the following is normal vaginal flora:
Lactobacillus
3 multiple choice options
what GPB is the most common cause of infection surrounding catheters, shunts, and prosthetic devices?
Corynebacterium jeikeium
3 multiple choice options
All of the following are general characteristics of the Streptococcus and Enterococcus genus, EXCEPT for:
White
3 multiple choice options
What would be the catalase result for Streptococcus and Enterococcus?
negative
1 multiple choice option
Select the correct order to perform ID for Streptococcus with alpha hemolysis:
Colonial morphology > Gram stain > catalase > optochin disk screen > bile solubility
3 multiple choice options
Select the correct order to perform ID for Streptococcus with beta hemolysis:
Colonial morphology > Gram stain > catalase > Lancefield grouping
3 multiple choice options
Select the correct order to perform ID for Streptococcus with no hemolysis:
Colonial morphology > Gram stain > catalase > PYR
3 multiple choice options
which of the following would be PYR +?
Streptococcus pyogenes
3 multiple choice options
which of the following alpha hemolytic organisms would give a susceptible result on an optochin disk screen?
Streptococcus pneumoniae
3 multiple choice options
which of the following would be PYR -?
Streptococcus gallolyticus
2 multiple choice options
which of the following organisms is beta hemolytic?
Streptococcus pyogenes
3 multiple choice options
which of the following alpha hemolytic organisms is resistant to optochin?
Streptococcus viridans
3 multiple choice options
which of the following is not a full-time pathogen?
Streptococcus gallolyticus
2 multiple choice options
which of the following is true of Lancefield grouping?
cannot be used for alpha-hemolytic samples
3 multiple choice options
which of the following is the correct order and amount of reagent to perform Lancefield grouping?
2 drops of rgt 1 + 2 drops of rgt 2 > inoculate test tube with organism > 4 drops of rgt 3
3 multiple choice options
all of the following are non-hemolytic, EXCEPT for:
Streptococcus agalactiae
3 multiple choice options
which of the following is responsible for rheumatic fever, sepsis, and impetigo?
Streptococcus pyogenes
3 multiple choice options
an organism has the following test results:
1. alpha-hemolytic
2. GPC
3. Catalase -
what tests would you run to complete identification?
optochin > bile solubility
3 multiple choice options
which cocci can sometimes be confused for a bacillus shape due to its elongated appearance?
Enterococcus
3 multiple choice options
what are the general colonial morphology characteristics of Streptococcus and Enterococcus?
grey, translucent, and hemolysis
2 multiple choice options
what would be the Gram stain arrangement for Streptococcus if done from a fluid?
chains or pairs
3 multiple choice options
which of the following is true of catalase?
breaks down hydrogen peroxide into water and oxygen
3 multiple choice options
what are the positive and negative controls for the catalase test?
Staphylococcus and Streptococcus
3 multiple choice options
which of the following is considered normal flora in the mouth?
streptococcus viridans
3 multiple choice options
what entry points do streptococci typically use to get to sterile sites?
colon or mouth
which test helps group strep by their cell wall antigenic properties?
lancefield grouping
3 multiple choice options
group A strep refers to which of the following organisms?
Streptococcus pyogenes
3 multiple choice options
what is the routine treatment for Group A strep?
penicillins
3 multiple choice options
which of the following is associated with scarlet fever signs?
S. pyogenes' pyrotoxic toxin
3 multiple choice options
which of the following components of S. pyogenes is responsible for beta hemolysis?
streptolysin O
3 multiple choice options
sore throat in children is usually caused by which of the following:
Streptococcus pyogenes
3 multiple choice options
which of the following is a complication from group A strep infection in children?
antibodies against strep can cross-react with heart tissue and cause permanent tissue damage
3 multiple choice options
which of the following complications can be caused by GAS?
if it breaks through the skin it can attack subcutaneous tissue causing cellulitis, causing muscle and fat destruction
3 multiple choice options
true or false: successful treatment for GAS necrotizing fasciitis is dependent on the swift administration of antibiotics
false
1 multiple choice option
when would you confirm S. pyogenes diagnosis with PYR?
if the sample was collected from a sterile site
3 multiple choice options
which of the following is normal flora in the female genital and lower GI tracts?
Streptococcus agalactiae
3 multiple choice options
which of the following organisms is associated with neonatal meningitis?
Streptococcus agalactiae
3 multiple choice options
administering penicillin to the mother prior to delivery will prevent transmission of which of the following to a newborn baby:
S. agalactiae
3 multiple choice options
which of the following organisms has Lancefield grouping "B"?
S. agalactiae
3 multiple choice options
which of the following is true of S. agalactiae?
susceptibility testing is not needed as it is universally susceptible to penicillin.
3 multiple choice options
Note 
Flashcards (22)