37. Examination of Stained Spermatozoa for Morphology - Cells Other than Spermatozoa in Semen Smears

What are other cells that may be present in semen smears?

1. red blood cells
2. leukocytes
3. squamous epithelial cells
4. germinative epithelial cells
5. bacteria

Describe red blood cells in semen samples

Red blood cells are rarely seen in semen. The sample may macroscopically show a red-tint

Describe leukocytes in semen samples

Leukocytes are present in cases of seminal vesiculitis, prostatitis, epididymitis or orchitis

Describe germinative epithelial cells in semen sample

Germinative epithelial cells includes multinucleated spermatids (seminiferous tubules), and ciliated “Medusa” cells (efferent tubules). They are observed during severe testicular dysfunction

Describe bacteria in semen samples

Bacteria should not be observed in fresh semen smears, but stored samples frequently show contamination.

Source of bacteria include contamination from artificial vagina, prepuce and penis.

True pathogens (Brucella abortus, Vibrio foetus, Trichomonas foetus) are usually overgrown in culture by saprophytic bacteria. Pathogenic streptococci can be present in stallion semen (venereal infection in mare)