Coenzymes & metabolic reactions (NOT DONE YET)

What are coenzymes and the classifications?

Coenzymes are …

thermo-labile , organic compounds loosely attached to enzyme.

Mainly water soluble Vit B and Vit C derivatives

Co enzymes can be classified into:

1. Co-dehydrogenase co enzymes (H carriers)

2. Group transferring co enzymes (Other groups than H)

** Eg:methyl/acyl/Co2/aldehyde

Co -dehydrogenase are used in what reactions and include?

Co hydrogenase are hydrogen carriers used in Redox reactions

They include:

1. Nicotinamide derived coenzymes (derived from Vit B3)

2. Flavin derived coenzymes (derived from Vit B2)

3. Gluathione

4. L-ascorbic acid (Vit C)

5. Coenzyme Q

6. Lipoic acid

Types of Nicotinamide derived coenzymes (Derived from Vit B3)

1. NAD (Nicotinamide Adenine Dinucleotide)

2. NADP (Nicotinamide Adenine Dinucleotide Phosphate)

3. Nicotinamide Mononucleotide (Co III) NMN

Niacin or Nicotinic acid (Name. Contains. what is converted into niacin)

Name: Vit B3 (Niacin or Nicotinic acid)

Contains: Pyridine ring

In body:

Tryptophan convert to Niacin.

Tryptophan 60mg = Niacin 1mg

Niacin or Nicotinic acid - bioactive coenzymes names functions and reduced form

Bio active coenzyme forms = NAD+ , NADP+

Functions:

NAD+ and NADP+ coenzymes in redox reaction.

Coenzyme undergoes reduction of pyridine ring by accepting hydride ion (H atom + electron)

Reduced forms:

NAD+ —> NADH + H+

NADP+ —> NADPH + H+

Flavin derived coenzymes (Derived from Vit B2) : Name, Bio active forms of Vit B2, How is FAD formed?

1. Name : Riboflavin (Vit B2)

2. Bio active forms: FAD (Flavin adenine dinucleotide), FMN (Flavin mononucleotide)

3. ATP— P—> FMN = FAD + …

Vit B2: FAD and FMN reduced forms and binding (with example)

1. FMN & FAD can reversible accept 2 H atoms forming FMNH2 or FADH2

2. FMN and FAD bound tightly to flavoenzymes that catalyse oxidation/reduction of substrate. (Eg: Succinate hydrogenase [FAD])

Gluathione - What is it

• Gluathione is tripeptide formed of glutamic acid, cysteine and glycine amino acid (y glutamyl cysteine glycine) G-SH

• Acts as hydrogen doner (2 G-SH ←→ G-S-SG)

L- absorbic acid (Name , and deficiency is called)

Name: Vit C (L- absorbic acid)

Deficiency disease = Scurvy

L-ascorbic acid ( functions + examples)

Main function: Reducing agent

Functions:

1. Coenzyme in hydroxylation reactions

• Example: hydroxylation of proline and lysine residues of collagen maintaining normal connective tissue and wound healing (Reduce easy bleeding)

2. Reduces ferric iron to ferrous form

• Absorption of dietary iron from intestine

Coenzyme Q and Lipoic acid

Co enzyme Q (Ubiquone)

1. Structurally similar to Vit K

2. Plays a role in electron transport and oxidative phosphorylation in respiratory chain

Lipoic acid

1. Its a member of Vit B group

2. Important for oxidative decarboxylation (eg:forms acetyl-coa from pyruvic acid or aKG→ S.CoA)

Group transferring coenzymes

Coenzymes transfer chemical groups other than hydrogen

1. Coenzyme A (CoA-SH) [contain pantothenic acid (Vit B5)]

2. Thiamine pyrophosphate (TPP) [Vit B1]

3. Pyridoxal phosphate [Vit B6]

4. Biotin [Vit B7]

5. Folic acid [Vit B9]

6. Cobalamine [Vit B12]

Pantothenic acid (Name , Functions, Example of structures)

Name: Vit B5 (Pantothenic acid)

Functions:

• coA contains thiol group (coA-SH) that carries acyl compounds

• Pantothenic acid component of acyl carrier protein of fatty acid synthase .

Structures: Succinyl coA, fatty acyl CoA, acetyl CoA

Pantothenic acid distribution and deficiency

Distribution : widely distributed but eggs, liver, and yeast most important sources

Deficiency: not well characterised in humans and no recommended daily allowance (RDA) established.

Thiamine (Name, Bio active form coenzyme function)

Name : Vit B1 (Thiamine)

Bio active form : TPP ( Thiamine + Pyrophosphate group from ATP)

Functions:

1. Transketolase reaction (imp for pentose synthesis from glucose)

2. Oxidative decarboxylation of a-keto acid

3. Oxidative decarboxylation of pyruvate & a-ketoglutarate which plays key role in energy metabolism of most cells & imp in CNS tissues

Pyridoxine (Name, Similarities of types, difference of types and types )

Name: Vit B6 (collective term of Pyridoxine, Pyridoxal, and Pyridoxamine)

Similarities: All Vit B6 types derived from pyridine and are precursors of PLP.

Diff: Types differ in nature of functional groups attached to rings

Types:

1. Pyridoxine = Plants

2. Pyridoxal = Food from animals

3. Pyridoxamine = Food from animals

PLP definition and functions

PLP is biologically active coenzyme called pyridoxal phosphate.

Functions (for enzymes catalyse reactions could involve amino acids):

1. Transamination (non essental amino acid)

2. Deamination

3. Decarboxylation (Histidine → Histamine + CO2)

4. Condensation (glycine + Succinyl CoA → δ aminolevulinic acid acid A (haem synthesis))

5. Glycogen phosphorylase (glycogenesis)

Biotin (Name, bond, found where and symptoms)

Name : Vit B7 (Biotin)

Bonded : Covalently bonded to ε-amino group of lysine residues in biotin-dependent enzymes

Found: large % requirement is supplied by intestinal bacteria

Biotin Deficiency incl symptoms and explanation

1. Antibiotic may cause deficiency in B7 → Hair loss/ depression/ dermatitis

Explanation : This is because large % of biotin supplied by intestinal bacteria

2. (Normal diet up to 20 eggs/day) Excess raw egg white as protein source → dermatitis glossitis, loss of appetite and nausea

Explanation: Raw egg white has glycoprotein avidin which tightly binds biotin and prevents absorption from intestine

Raw egg may cause Salmonella infection

Folic Acid (Name, Key roles, Source)

Name: Vit B9

Key roles :

1C metabolism which is essential for biosynthesis of several compounds

Folate (as N5-methyl-THF) acts as the methyl donor for converting homocysteine → methionine.

Folic acid (Deficiency)

Deficiency:

Most common in US (especially among preg woman and alcoholics)

Primary result is Megablastic anemia

Caused by diminished synthesis of purines and TMP → Inability of cells (incl RBC precursor) to make DNA & inability to divide.