MOLBIO - Practical Exam 2

FALSE

TRUE OR FALSE

Personal protective equipment can be modified and should be worn correctly for the duration of work in potentially contaminated areas.

NIOSH-certified N95 respirator

What personal protective equipment blocks the entry of aerosol transmitted pathogens?

Doffing

It is a high-risk process that requires a structured procedure, and a designated area to reduce the possibility of self-contamination or other exposure.

High-efficiency particulate air, High efficiency particulate air

What does HEPA in HEPA filter stands for?

FALSE

TRUE OR FALSE

Biological safety cabinet Class 2 type B allows air exhausted into the room after passing through HEPA filters.

biosafety

Prevents unintentional release or exposure to pathogens and toxins.

biosecurity

Prevents intentional release or exposure to pathogens and toxins.

SERIOUS

Identify the severity and scoring of the following:

Severity

3

Identify the severity and scoring of the following:

Scoring

5

Identify the risk matrix score if the severity causes patient death and frequency is improbable.

moderate risk

RISK MATRIX SCORE - 5

What is the associated risk level?

Polymerase Chain Reaction Cabinet

Identify equipment

Reagent preparation room

What section of the molecular lab is it located?

Amplification detection room

Which section is this equipment located?

negative pressure

What is the airflow for this section?

Specimen Processing Lab-> Amplification Lab

What is the Sample Flow for specimens received in a PCR laboratory?

Uracil-N-Glycosylase

What decontamination measure selectively destroy PCR products but not the template DNA through incubation?

Level 2

What is the biosafety level of the CDUH molecular laboratory?

Biosafety cabinet class II

Identify equipment specifically used in the molecular laboratory

Specimen Preparation RoomS

Which section is this located?

Master Mix Preparation

What is prepared in this area of the molecular lab to reduce the number of maneuvers thus preventing contamination?

FALSE

TRUE OR FALSE

This area can be combined with the postamplification room.

real time reverse transcriptase polymerase chain reaction

What specific type of PCR is used in viral RNA detection of SARS-CoV-2?

negative pressure

What would be the airflow in the area for nucleic acid extraction?

10% sodium hypochlorite

What are the agents used in decontamination using bleach?

2,500/2500

Based on the volume shown above, using a P1000 how many microliter should be aspirated?

aerosol-resistant tip

When using air-displacement pipette in molecular laboratory what equipment should be added?

NO

YES OR NO

Refer to the image above. Is this acceptable in aspirating sample?

air-displacement pipette

What micropipette is used in which the piston does not come in contact with the liquid?

oropharyngeal swab collection

Identify the specimen collection with the image above

tonsillar area

Give one area to be swab with the collection above

B

Refer to the image above. Which is not suitable for NPS collection?

True

TRUE OR FALSE

Only one swab is needed for NPS collection when collecting from both nostrils.

Yes

:YES OR NO

Refer to the image above disregard the direction of the arrow. Is this acceptable in dispensing a sample?

positive displacement pipette

What micropipette is used in which the piston comes in contact with the liquid?