TRIGLYCERIDES cHLORIDE

Triglycerides

Mainly provide energy for cells and constitute 95% of tissue storage fat.

Clinical conditions associated with high triglycerides

Atherosclerosis, lipid metabolism disorders, lipase activity deficiency, apolipoprotein-CII deficiency, diabetes, renal disease, endocrine disorders.

Elitech method for triglycerides

An enzymatic-colorimetric, end-point method to measure triglyceride concentration.

Key reagents in Elitech triglyceride method

Pipes buffer, ATP, ADPS, amino-4-antipyrine, magnesium salt, various enzymes.

Precautions for triglyceride reagents

Sodium azide is present; dispose carefully, use clean equipment, and follow Good Laboratory Practices.

Triglyceride sample collection

Fasting patients' serum or lithium heparinized plasma, store under specified conditions.

Formula for triglyceride concentration

Triglycerides = Asample / Astandard × standard concentration (n).

NCEP reference values for triglycerides

Normal <150 mg/dL, borderline high 150-199 mg/dL, high 200-499 mg/dL, very high ≥500 mg/dL.

Common interferences in triglyceride measurement

Bilirubin, hemoglobin, ascorbic acid, glucose, uric acid, N-Acetyl-Cysteine, paracetamol metabolites.

Classical principle of chloride determination

Combines chloride with silver or mercury to form compounds; uses titrimetric and colorimetric methods.

Elitech method for chloride measurement

Mercuric thiocyanate colorimetric method measured at 500 nm.

Reagents in chloride Elitech method

Mercuric thiocyanate, ferric nitrate, mercuric nitrate in methanol and water.

Precautions for chloride reagents

Handle toxic and caustic substances carefully, store at 15-25°C away from light.

Handling chloride samples

Minimize stasis during collection, separate quickly, and dilute urine samples.

Calculation of chloride concentration

Chloride = Asample / Astandard × standard concentration (n).

Expected values for chloride

Serum/Plasma: 98–107 mEq/L, CSF: 118–132 mEq/L, Urine: 110–250 mEq/24h.

Quality control in chloride testing

Use ELITROL I and II for normal and abnormal control checks.

Limitations of the chloride method

Does not follow Beer’s Law, linear range from 70–120 mEq/L, requires calibration curve verification.