Chapter 18 - Biotechnology 

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Biotechnology tools

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are being used for a variety of purposes, including cancer therapies, increased agricultural yields, gene therapies for genetic disorders, de- extinction projects (such as the one to reintroduce the woolly mammoth), and extinction projects (such as attempting to eliminate pathogens that cause human disease), to name a few.

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Gel electrophoresis

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is a method for separating DNA fragments based on size and charge.

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19 Terms

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Biotechnology tools

are being used for a variety of purposes, including cancer therapies, increased agricultural yields, gene therapies for genetic disorders, de- extinction projects (such as the one to reintroduce the woolly mammoth), and extinction projects (such as attempting to eliminate pathogens that cause human disease), to name a few.

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Gel electrophoresis

is a method for separating DNA fragments based on size and charge.

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Bacteriophages

are viruses that may infect bacteria.

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positive cathode

A(n) is attached to the gel's bottom, while a negative anode is attached to the gel's top.

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Cas9 enzyme

The may be directed to cut at a specific DNA sequence by employing synthesized guide RNA bits that correspond to the intended site of the cut to be produced.

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DNA

An electric current is supplied to the gel after samples are put into wells at the top of the gel.

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CRISPR

In nature, the clustered regularly interspaced short palindromic repeats ()- Cas9 system serves as an adaptive immune system in bacteria.

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PCR

includes cycles of DNA replication utilizing primers specific to the beginning and end of the fragment of the DNA sequence to be amplified.

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gel

The is typically constructed of agarose and has minute holes through which the DNA fragments flow.

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selectable marker

A(n) is required for the foreign plasmid DNA so that cells that have integrated the plasmid DNA can be identified.

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Phosphate groups

are somewhat negatively charged.

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new virus

When a(n) infects the bacteria, the bacteria compare the DNA sequences from the to those stored in the bacterium.

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viral DNA

When a bacteriophage infects a bacterium, the is broken up and inserted between short palindromic repeats in the bacteria's DNA.

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Heat shock

is one way of converting bacteria in which bacterial cells are combined with foreign DNA and then exposed to heat.

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abundance of phosphate groups

Because of the in DNA molecules, they carry a net negative charge.

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Bacterial transformation

involves the introduction of foreign DNA into bacterial cells.

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CRISPR

has the ability to modify DNA sequences.

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Cas9 enzyme

To determine where to cut DNA, the employs a bit of guide RNA.

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PCR

may be used to replicate a particular DNA segment millions of times.