STATE CONTAGIOUS QUIZ

# EMB (Eosin Methylene Blue) Agar **Purpose:** Selective and differential medium. * **Selective:** Inhibits Gram-positive bacteria. * **Differential:** Differentiates lactose fermenters. ### Green metallic sheen * Strong lactose fermentation * Classic organism: **E. coli** ### Colony colors * **Green metallic sheen:** Strong lactose fermenter (E. coli) * **Purple/black colonies:** Lactose fermenter (Enterobacter, Klebsiella) * **Colorless colonies:** Non-lactose fermenter (Salmonella, Shigella) --- # MacConkey Agar ### Contains * Lactose * Neutral red indicator * Crystal violet * Bile salts ### Purpose * Selects for Gram-negative bacteria * Differentiates lactose fermenters ### Results **Pink/red colonies** * Lactose fermenter **Colorless colonies** * Non-lactose fermenter --- # TSIA (Triple Sugar Iron Agar) ### Tests for * Glucose fermentation * Lactose fermentation * Sucrose fermentation * Gas production * Hydrogen sulfide (H₂S) ### Sugars present * Glucose (0.1%) * Lactose (1%) * Sucrose (1%) ### Color meanings Yellow = Acid (A) Red = Alkaline (K) Black = H₂S production Cracks/lifting = Gas production ### Interpretations **A/A** * Yellow slant * Yellow butt * Ferments glucose + lactose and/or sucrose **K/A** * Red slant * Yellow butt * Ferments glucose only **K/K** * Red slant * Red butt * No sugar fermentation ### Proper inoculation * Stab the butt once. * Streak the slant as you remove the needle. --- # Mannitol Salt Agar (MSA) ### Purpose Selective and differential. ### Selective * 7.5% salt selects for **Staphylococcus**. ### Differential * Mannitol fermentation ### Results Yellow media * Mannitol fermented * Acid produced Red/Pink media * No mannitol fermentation ### Species **Staphylococcus aureus** * Yellow * Pathogenic **Staphylococcus epidermidis** * Pink/red * Usually non-pathogenic --- # Blood Agar ### Classification **Both enriched and differential** ### Hemolysis **Beta** * Complete hemolysis * Clear zone **Alpha** * Partial hemolysis * Green zone **Gamma** * No hemolysis * No color change --- # Kirby-Bauer Test Purpose: Determine antibiotic susceptibility. ### Measure Diameter of the **zone of inhibition** (mm). Large zone * Sensitive Small/no zone * Resistant --- # UV Light ### Mechanism UV damages DNA by forming **thymine dimers**. ### Most harmful wavelength **254 nm (UV-C)** ### Interpretation Less bacterial growth = UV more effective. --- # Urease Test ### Purpose Detect urease enzyme. ### Reaction Urea → Ammonia + CO₂ Ammonia raises pH. ### Color Pink = Positive Yellow/orange = Negative ### Strong urease-positive genus **Proteus** --- # Catalase Test ### Reagent Hydrogen peroxide (3%) ### Positive Bubbles immediately ### Negative No bubbles Reaction: 2H₂O₂ → 2H₂O + O₂ The bubbles are oxygen gas. --- # Passive (Indirect) Agglutination ### Definition Antibodies bind to antigen-coated particles, causing visible clumping. Positive = Clumping Negative = Smooth suspension --- ## High-Yield Organisms to Memorize | Organism | Key Feature | | ------------------------------ | ------------------------------------------------- | | **E. coli** | Green metallic sheen on EMB | | **Proteus** | Strong urease positive | | **Staphylococcus aureus** | MSA turns yellow (mannitol fermenter) | | **Staphylococcus epidermidis** | MSA stays pink | | **Salmonella** | Non-lactose fermenter; often H₂S positive on TSIA | | **Shigella** | Non-lactose fermenter; H₂S negative | ## Last-minute memorization * **EMB** → Green sheen = **E. coli** * **MacConkey** → Pink = lactose fermenter * **MSA** → Yellow = mannitol fermenter (**S. aureus**) * **Blood agar** → Beta = clear, Alpha = green, Gamma = none * **TSIA** → Yellow = acid, Red = alkaline, Black = H₂S * **Kirby-Bauer** → Bigger zone = more susceptible * **Urease** → Pink = positive (**Proteus**) * **Catalase** → Bubbles = positive * **UV** → 254 nm causes thymine dimers

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Chapter 10: Stress, Health, and Coping Stress vs. Stressor * Stressor: Anything that causes stress (an event, situation, or demand). * Example: Exams, losing a job, relationship problems. * Stress: The body’s response to a stressor. * Example: Feeling anxious, increased heart rate. Types of Stressors 1. Catastrophe * Large, unpredictable events affecting many people. * Example: Natural disasters, wars. 2. Significant Life Changes * Major events that require adjustment. * Example: Divorce, moving, starting college. 3. Daily Stressors * Small everyday problems. * Example: Traffic, homework, arguments. Sympathetic Nervous System & Fight-or-Flight * Activates during stressful situations. * Prepares the body to react. * Causes: * Increased heart rate * Faster breathing * More energy released General Adaptation Syndrome (GAS) A three-stage response to stress: 1. Alarm: Body reacts to stress. 2. Resistance: Body tries to adapt and continue functioning. 3. Exhaustion: Long-term stress drains resources. Type A vs. Type B Personality Type A: * Competitive * Impatient * Time-urgent * More stress-prone Type B: * Relaxed * Patient * Less stressed Immune System & Stress * Long-term stress weakens the immune system. * Makes the body more vulnerable to illness. Locus of Control Internal locus of control: * Belief that you control your outcomes. External locus of control: * Belief that outside forces control outcomes (luck, fate). Managing Stress * Aerobic exercise * Meditation * Relaxation techniques * Spending time with pets * Social support * Healthy sleep habits ⸻ Chapter 11: Social Psychology Fundamental Attribution Error * Tendency to explain others’ behavior by personality instead of situations. * Example: “He’s lazy” instead of considering he may be exhausted. Attitudes * Feelings and beliefs that influence behavior. * Made of: * Thoughts * Feelings * Actions Cognitive Dissonance * Mental discomfort from having conflicting beliefs or behaviors. * Example: Knowing smoking is unhealthy but continuing to smoke. Social Contagion * Spread of behaviors, emotions, or ideas through groups. * Example: Panic spreading through a crowd. Classic Studies Asch’s Line Study * Showed people may conform to a group even when the group is wrong. Milgram’s Obedience Study * Showed people may obey authority figures even when uncomfortable. Social Facilitation vs. Social Loafing Social Facilitation: * Performance improves when others are watching. Social Loafing: * People put in less effort when working in groups. Deindividuation * Losing self-awareness and responsibility in a group. * Example: Crowds acting differently than individuals. Ingoups vs. Outgroups Ingroup: * Group you belong to. Outgroup: * Group you see as different. Can lead to: * Bias * Stereotypes * Prejudice Passionate vs. Companionate Love Passionate Love: * Intense attraction and excitement. Companionate Love: * Deep affection, trust, and commitment. Altruism & Bystander Effect Altruism: * Helping others without expecting a reward. Bystander Effect: * People are less likely to help when others are present. ⸻ Chapter 12: Personality Personality * Patterns of thoughts, feelings, and behaviors that make someone unique. Psychoanalytic Theory (Freud) Id * Wants immediate pleasure. * “I want it now.” Ego * Reality-based decision maker. * Balances id and superego. Superego * Morals and rules. Defense Mechanisms Ways the mind protects itself from anxiety. Examples: * Denial: Refusing to accept reality. * Projection: Blaming feelings on others. * Rationalization: Creating excuses. Humanistic Theory * Focuses on personal growth and reaching full potential. * Carl Rogers developed Person-Centered Therapy. Trait Theory (Big Five) Five major personality traits: OCEAN * Openness * Conscientiousness * Extraversion * Agreeableness * Neuroticism Spotlight Effect vs. Narcissism Spotlight Effect: * Believing others notice you more than they actually do. Narcissism: * Excessive self-focus and feeling superior. Kruger-Dunning Effect * People with low ability may overestimate their skills. Individualistic vs. Collectivistic Cultures Individualistic: * Focus on independence and personal goals. Collectivistic: * Focus on family, groups, and cooperation. ⸻ Chapter 13: Psychological Disorders Psychological Disorder * A pattern of thoughts, feelings, or behaviors that causes distress or problems functioning. Anxiety Disorders * Excessive fear or worry. Examples: * Generalized anxiety disorder * Panic disorder * Phobias OCD * Obsessions (unwanted thoughts) * Compulsions (repetitive behaviors) PTSD * Stress disorder after experiencing or witnessing trauma. Substance Use Disorder * Difficulty controlling substance use despite negative effects. Major Depressive Disorder Symptoms include: * Persistent sadness * Loss of interest * Changes in sleep or energy Bipolar Disorder * Episodes of depression and mania. * Mania involves unusually high energy, mood, or activity. Suicidal Ideation * Thoughts about suicide. (Important: any concerns about safety should be taken seriously and support should be reached out to.) Schizophrenia * Disorder involving: * Hallucinations * Delusions * Disorganized thinking Personality Disorders Antisocial PD * Disregard for others’ rights and social rules. Narcissistic PD * Extreme need for admiration and lack of empathy. Borderline PD * Difficulty with emotions, relationships, and sense of self. ⸻ Chapter 14: Treatment Psychotherapy * Treatment using psychological methods to improve mental health. Types of Psychotherapy Psychoanalysis/Psychodynamic * Focuses on unconscious conflicts and past experiences. Client-Centered/Humanistic * Focuses on acceptance, growth, and self-understanding. Behavioral Therapy * Changes behaviors through learning principles. Cognitive Therapy * Changes unhealthy thought patterns. Cognitive Behavioral Therapy (CBT) * Combines changing thoughts and behaviors. Family Therapy * Works with family relationships. Group Therapy * Therapy with multiple people. Evidence-Based Practice * Treatments supported by scientific research. Therapeutic Lifestyle Change Improving mental health through: * Exercise * Sleep * Nutrition * Social connection Biomedical Treatments Medication * Treats symptoms through biological processes. ECT (Electroconvulsive Therapy) * Uses controlled electrical stimulation to treat severe depression. Psychosurgery * Surgery involving the brain (rare). TMS (Transcranial Magnetic Stimulation) * Uses magnetic stimulation to affect brain activity. ⸻ Quick Test Tips: * Stress = response, Stressor = cause * Asch = conformity * Milgram = obedience * Big Five = OCEAN * Id = pleasure, Ego = reality, Superego = morals * CBT = thoughts + behaviors * Bystander effect = less helping in groups

Thẻ ghi nhớ: wf của ADD ,PHARMACY , EFFECT, CONTAGION,VEIL,NOTE,WILD | Quizlet

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