Studied by 0 people
Looks like no one added any tags here yet for you.
Electrophoresis
A separation technique that involves the migration of charged particles in an electrical field.
Cations
Positively charged particles that move towards the negatively charged electrode (cathode).
Anions
Negatively charged particles that move toward the positively charged electrode (anode).
Densitometer
An instrument used to quantitate how much protein is present in each band after separation.
Cellulose acetate
A medium commonly used for performing electrophoresis.
Albumin
The primary type of protein, typically migrating the fastest in serum protein electrophoresis.
Globulins
A category of proteins in serum protein electrophoresis, which includes alpha-one, alpha-two, beta, and gamma globulins.
M spike
An increased amount of a particular immunoglobulin seen in cases like monoclonal gammopathy.
Monoclonal gammopathy
A condition where a single clone of plasma cells produces a large amount of a particular immunoglobulin.
Immunofixation electrophoresis (IFE)
A method that combines electrophoresis and fixation to identify specific antigens or antibodies.
Antigen-antibody sandwich
A structure formed in immunochromatography tests where an antigen is bound between two antibodies.
Mobile phase
The phase in chromatography that moves across the stationary phase and carries the components being separated.
Stationary phase
The phase in chromatography that remains fixed and is where the separation of components occurs.
Qualitative test
A test that determines the presence or absence of a particular component without measuring quantity.
Quantitative test
A test that measures the amount or concentration of a particular component.
Vertical flow assays
Tests where the sample flows in a vertical direction, typically yielding faster results.
Lateral flow assays
Tests where the sample flows laterally across the membrane, commonly used for rapid tests.
Chromatography
A technique used to separate complex mixtures based on different physical interactions.
Mass spectrometry
An additional technology that can be combined with chromatography for quantification purposes.
Thin layer chromatography (TLC)
A type of chromatography that uses a stationary phase that is applied to a flat surface.
High Performance Liquid Chromatography (HPLC)
A type of liquid chromatography that uses high pressure to force the sample through the stationary phase.
