microbio lab final

Balantidium coli- cyst

Giardia lamblia (intestinalis)-cyst and trophozoite

Trypanosoma gambiense

Trichomonas vaginalis

Plasmodium sp.  

Taenia solium (egg) 

Schistosoma mansoni (egg)

Enterobius vermicularis (egg)

Enterobius vermicularis (adult) 

Dirofilaria immitis (adult)

Diphyllobotrium latum (egg)

Candida albicans

Trichophyton mentagrophytes

 S. pyogenes

S. pneumoniae

 S. aureus

S. epi

N. gonorrhoea

GC smear

T. pallidum

MAC

Selects for gram neg (growth = gram neg)

Pink/purple CFU = pos. lactose fermentation

MSA

Selects for gram pos. halophiles (salt-tolerant/halotolerant bacteria)

• staphylococcus 

• micrococcus

• bacillus

• enterococcus

Differential based on ability to ferment mannitol

Yellow = pos. mannitol fermentation 

BA

Used for the growth of fastidious microbes (microorganisms difficult to cultivate due to complex or limited nutritional requirements)

Differential through hemolysis

• Alpha-hemolysis: partial hemolysis, produces a green-yellow zone of the media around the

bacterial colony.

• Beta-hemolysis: complete hemolysis, produces a clearing of the media around the bacterial

colony.

• Gamma-hemolysis: no hemolysis, no change to the media around the bacterial colony.

Alpha-hemolysis

partial hemolysis, produces a green-yellow zone of the media around the

bacterial colony

Beta-hemolysis

complete hemolysis, produces a clearing of the media around the bacterial colony

Gamma-hemolysis

no hemolysis, no change to the media around the bacterial colony

Starch hydrolysis

Positive: clearing around growth

Neg.: no clearing

Simmons citrate

Purpose: identify ability to utilize citrate as carbon and energy source

SIM

Purpose: identify motility, indole production, hydrogen sulfide production 

Lactose/Mannitol (phenol red) broth

Purpose: identify lactose/mannitol fermentation

MRVP

Purpose: Identify glucose fermentation pathway: mixed acid or butanediol

MR = methyl red 

• identifies mixed acid pathway

• req addition of methyl red

VP = voges-proskauer test

• identifies butanediol pathway

• req alpha naphthol and KOH

Snyder media

tests susceptibility to dental caries

Yellow/orange after 24 & 48 hrs =pos/high susceptibility

Green after 24 & 48 hrs =neg/low susceptibility