Types of Microscopes and Uses
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13 Terms
Brightfield microscope (standard)
Specimen is dark, background is light. Good for stained material!
Darkfield microscope
Specimen is light, background is dark. Good for observing LIVE motile cells.
Phase contrast microscope
Good for observing INTERNAL structures
Electron microscope
High resolution and magnification. Can observe viruses!
Transmission Electron microscope (TEM)
Thin section of specimen. Used to study organelles within a cell!
Scanning Electron microscope (SEM)
3D view of specimen
What objective lens has the smallest field of view?
High Power 40x
Which objective lens has the widest field of view?
Scanning lens 4x
The magnifying lens closest to the object being viewed with the microscope is the
Oil immersion
Define the term parfocal?
One lens is focus, the others as well
Why is the resolving power of an microscope so spectacular?
It can observe viruses
What type of microscope can be used to observe the motility of Vibrio cholerae, the organism that causes cholera?
Darkfield microscope
Why is a smaller resolving power value better than a larger one?
It can be seen by the RP formulation for wavelength of illumination