Studied by 18 peoplewhat is iodine an indicator of?
carbohydrates
what color do carbohydrates turn when exposed to iodine?
brown/black
what does a microscope do to an image?
inverts & reverses it
how to calculate total magnification of a microscope
magnification of ocular x objective
what is magnification
making an image appear larger
what is resolution
the clearness of an object
objective lenses
on the revolving nosepiece
gives 2nd magnification
stage
where specimen/slide is placed
TEM
transmission electron microscope
total magnification = 200,000x
views interior structures
SEM
scanning electron microscope
total magnification = 100,000x
views 3D surface of objects
what do you use methylene blue on?
to see cells & nucleus of cheek cells
what should you use iodine on?
to see the cell wall of onion cells
how to make a wet mount
put a drop of fluid on slide, put coverslip on edge of fluid at a 45 degree angle.
how to determine objective magnification (if total magnification & ocular are known)
total magnification ÷ ocular = objective magnification
ocular lens
magnifies
what you look through
coarse adjustment
moves stage up or down
fine adjustment
focuses on image to get clarity/resolution
how to hold microscope
hold arm & base
mirror
reflects the light
base
supports microscope
relationship between resolution & magnification
when resolution goes down, magnification goes down
low power
more specimen, less detail
what do TEM & SEM have in common
they’re both used on dead organisms
what happens when magnification is higher
higher the magnification, the more detail & less of the object
lower the magnification, the less detail & more of the object
Note
Flashcard