microscopes quiz

what is iodine an indicator of?

carbohydrates

what color do carbohydrates turn when exposed to iodine?

brown/black

what does a microscope do to an image?

inverts & reverses it

how to calculate total magnification of a microscope

magnification of __ocular__ x __objective__

what is magnification

making an image appear larger

what is resolution

the clearness of an object

objective lenses

* on the revolving nosepiece


* gives 2nd magnification

stage

where specimen/slide is placed

TEM

* transmission electron microscope
* total magnification = 200,000x
* views interior structures

SEM

* scanning electron microscope
* total magnification = 100,000x
* views 3D surface of objects

what do you use methylene blue on?

to see cells & nucleus of __cheek cells__

what should you use iodine on?

to see the cell wall of __onion cells__

how to make a wet mount

put a drop of fluid on slide, put coverslip on edge of fluid at a __45 degree angle__.

how to determine objective magnification (if total magnification & ocular are known)

total magnification ÷ ocular = objective magnification

ocular lens

* magnifies
* what you look through

coarse adjustment

moves stage up or down

fine adjustment

focuses on image to get clarity/resolution

how to hold microscope

hold arm & base

mirror

reflects the light

base

supports microscope

relationship between resolution & magnification

when resolution goes down, magnification goes down

low power

more specimen, less detail

what do TEM & SEM have in common

they’re both used on dead organisms

what happens when magnification is higher

* higher the magnification, the more detail & less of the object
* lower the magnification, the less detail & more of the object