Exam 2

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Phase 1 Clinical Trials (1 year)

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Phase 1 Clinical Trials (1 year)

  • healthy human volunteers. Small short studies done in a few subject (<20)

  • test escalating single doses and multiple doses

  • pharmacodynamic and pharmacokinetic studies

  • safety and tolerance are the endpoints- does the drug mess with the person’s health

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Phase 2 Clinical Trials (2 years)

  • several hundred patients with the target disease. Can involve multiple institutions

  • placebo controlled, double-blind study designs are the gold standard

  • Phase 2a: proof of concept. Do patients improve? Is the drug safe?

  • Phase 2b: dose response studies. Is it better? Is it safe? (nausea, headache, gastrointestinal side effects, liver function tests).

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Phase 3 Clinical Trials (3-5 years)

  • Hundreds to thousands of patients with disease. Definitely involves multiple institutions.

  • Placebo controlled, double blind study designs are the gold standard

  • Must show clinical efficacy. This is a key point in drug development

  • Many drugs die at this point although the company has already invested millions of dollars

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Concurrent work with Phase 3 Clinical Trials (3-5 years)


  • Drug interaction-studies in healthy volunteers

  • Long-term toxicology studies: chronic and reproductive. Is it teratogenic?

  • Manufacturing considerations

  • Preparing the market

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<p>Alcohol Pharmacodynamics </p>
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<p>Alcohol Pharmacodynamics </p>

Alcohol Pharmacodynamics

  • GABA(A) Agonist

  • Increase intracellular Cl- (higher concentration outside then inside flow through GABA (A)) 


  • CNS depressant (increase of intracellular chlorine in the cell) 

  • Very poor potency 

  • Around 14g/EtOH/drink

  • Most drugs=mg-µg quantities 

  • BAL of 0.1%=100mg/100ml of blood (legal=0.08) 

  • Mempredine (opiate) high micromolar low mammolar. EtOH high millimolar concentration (10-4). 


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Alcohol Pharmacokinetics- Absorption

  • Absorbed into partial blood from stomach and intestines 

  • Slow absorption into system

  • Long latency 

  • Large 1st pass effect 

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<p><strong><span style="font-family: Arial, sans-serif">Alcohol Pharmacokinetics- Distribution</span></strong></p><p></p>
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<p><strong><span style="font-family: Arial, sans-serif">Alcohol Pharmacokinetics- Distribution</span></strong></p><p></p>

Alcohol Pharmacokinetics- Distribution

  • Small, uncharged molecule 

  • Easily gets across BBB

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<p><strong><span style="font-family: Arial, sans-serif">Alcohol Pharmacokinetics- Metabolism</span></strong></p>
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<p><strong><span style="font-family: Arial, sans-serif">Alcohol Pharmacokinetics- Metabolism</span></strong></p>

Alcohol Pharmacokinetics- Metabolism

  • Processed by 2 different liver enzymes:

  • ADH (alcohol dehydrogenase) 

  • P450 (2E1)

  • ALDH

  • Know ethanol is produced by alcohol dehydrogenase and P450 

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<p>Alcohol tolerance, metabolism and liver injury </p>
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<p>Alcohol tolerance, metabolism and liver injury </p>

Alcohol tolerance, metabolism and liver injury

Breaking alcohol by P450 to ADH. to acetic acid. There is a different amount, chromic user will have a different latency and concentration in their blood than a person who doesn't drink. Fatty liver first then alcoholic hepatitis then leads to cirrhosis

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rate of elimination

 is constant per unit time: alcohol follows zero order elimination kinetics 

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<p>Alcohol: time vs. concentration</p>
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<p>Alcohol: time vs. concentration</p>

Alcohol: time vs. concentration

Plotting concentration in a function time, you will have a straight slope. Horizontal doesn't change because alcohol stays the same as a zero order kinetic drug.

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<p>Alcohol Effects on the brain </p>
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<p>Alcohol Effects on the brain </p>

Alcohol Effects on the brain

GABA (A) chloride channel!!!


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<p>GABA(A) effect with alcohol</p>
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<p>GABA(A) effect with alcohol</p>

GABA(A) effect with alcohol

GABA(A) agonist, once it binds cause influx of chloride and have a hyperpolarized membrane (increases negative value). Adding etOH bind to a specific part and cause influx of chloride and hyperpolarize past the -70mV. 


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<p>Ethanol Discriminating protocol experiment </p>
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<p>Ethanol Discriminating protocol experiment </p>

Ethanol Discriminating protocol experiment

Rats and mice can discriminate a reinforcing property of the EtOH as they prefer EtOH over sugar water.

A lever press, a drop comes out and they measure how many times they pick the lever and how each lever affects the mouse. 


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<p>No drug treatment vs picrotoxin </p>
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<p>No drug treatment vs picrotoxin </p>

No drug treatment vs picrotoxin

Mouse press 25x on sugar. They prefer EtOH 3:1 to sugar water with the no drug treatment. We know they GABA(A) (picrotoxin) blocks GABA(A) receptor and wr see no difference with the 2. GABA A antagonist, the mouse does not tell the difference between etOh and sugar water since GABA A is blocked. 


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<p>Alcohol effecting amygdala </p>
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<p>Alcohol effecting amygdala </p>

Alcohol effecting amygdala

The amygdala is the primary site of action for EtOH’s behavior and reinforcing (drug seeking) effects. These actions occur at lower doses of EtOH. High doses have more generalized effects on more diverse brain areas (motor function etc).

  • The amygdala is involved in perception of stressful conditions and responses to fear and stress

Lowest dose of alcohol affects the amygdala in our brian (starts here). Interneuron is a GABA and the receptor for this internourn is a projection neuron. Once GABA binds to receptors will cause decrease activity in that neuron. 


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Alcohol and Anxiety

  • Amygdala involved in perception of stressful conditions and responses to fear/stress

  • Alcohol increases GABA activity and decreases output of downstream neurons leading to depressed fear response 

  • High anxiety promotes alcohol drinking behavior, to become less stress, cause decrease in activity (calming feeling) but there is a population that have a genetic predisposition, once undergo anxiety it will reinforce itself and if you continue then you get frustrated and stopped and causes withdrawal which induce anxiety which makes you want to drink more which leads to addiction. The root is using alcohol to deal with anxiety and the GABA activity give you  a depress fear response but does not help solve the stressful situation/


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<p><strong><span style="font-family: Arial, sans-serif">Molecular Pathway of Stress Relief&nbsp;</span></strong></p><p></p>
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<p><strong><span style="font-family: Arial, sans-serif">Molecular Pathway of Stress Relief&nbsp;</span></strong></p><p></p>

Molecular Pathway of Stress Relief 

  • Neuropeptide Y (NPY)= anti-stress peptide

  • Corticotropin releasing factor (CRF)= pro-stress peptide

  • CRF binding to its receptor causes increase in NPY production (through activation of PKA and KREB)

  • CRF is released and bind to a GPCR receptor. Galpa binds CRF and activates and Galpha dissociate and active adenylyl cyclase to cyclic AMP then bind to PKA (inactive molecule) dissociate inhibitor then leave to inactivate PKA to nucleus and activates KREB and it will target to their target. Cyclic amp blinds to cyclic CBP. translate to NPY (increase inn system)

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<p>Stress Pathway (with negative feedback loop (-) </p>
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<p>Stress Pathway (with negative feedback loop (-) </p>

Stress Pathway (with negative feedback loop (-)

NPY shuts production of CRF during the negative feedback so it doesn't rapid increase anxiety. 

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<p>Stress Pathway (chronic ethanol) </p>
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<p>Stress Pathway (chronic ethanol) </p>

Stress Pathway (chronic ethanol)

Disrupting NPY when drinking (prevent NPY production) 

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<p>Drug Tolerance (decreases in sensitivity to a drug after repeated administration)  </p>
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<p>Drug Tolerance (decreases in sensitivity to a drug after repeated administration)  </p>

Drug Tolerance (decreases in sensitivity to a drug after repeated administration)

  • Tolerance is a state of reduced drug sensitivity

  • Occurs as an adaptive response to the presence of a drug

  • Tolerance is an effort to maintain homeostasis 

  • Removal of a drug disturbs the new homeostatic level (withdrawal)  

  • Need more drug to produce the same effect.

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<p>Pharmacokinetic Tolerance </p>
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<p>Pharmacokinetic Tolerance </p>

Pharmacokinetic Tolerance

Similar latency time between the two (it doesn't change absorption of the drug, but change metabolism. You will have less concentration in chronic user. 

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<p>Mechanism of Pharmacokinetic tolerance </p>
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<p>Mechanism of Pharmacokinetic tolerance </p>

Mechanism of Pharmacokinetic tolerance

Have higher levels in the liver with chronic user of alcohol so they can metabolize alcohol quickly

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<p>Acute Ethanol Tolerance </p>
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<p>Acute Ethanol Tolerance </p>

Acute Ethanol Tolerance

Ethanol tolerance occurs quickly (within hours after a single exposure) and is demonstrated by behavioral assessments at times when blood alcohol levels are rising and falling but are at equal levels. 

At distent and intent when alcohol match. Have more anxiety when alcohol was being broken down. Anxiety is higher one the down turn. 

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<p>Normal function of a synapse between an amygdala projections neuron and a neuron in the PFC </p>
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<p>Normal function of a synapse between an amygdala projections neuron and a neuron in the PFC </p>

Normal function of a synapse between an amygdala projections neuron and a neuron in the PFC

Calcium is higher outside and once it comes in and glutamate release in synapse and NMDA and AMPA receptor targets.

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<p>Function of a nerve terminal in the presence of ethanol </p>
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<p>Function of a nerve terminal in the presence of ethanol </p>

Function of a nerve terminal in the presence of ethanol

Hyperpolarizes the membrane and no release of glutamate due to high chloride levels. Without glutamate being released then you have an increased amount of glucose in the presynaptic neuron. Then it makes more glutamatergic vesicles and increases the number of receptors but only NMDA and AMPA stays the same. This is the response to ethanol in the system. 


Stop administering ethanol, everything gets released. Have an influx of calcium and AP activates and a lot of glutamate release with a lot of receptors with a high excitatory response. (headaches, anxiety withdrawal) can also cause seizures 

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<p><strong><span style="font-family: Arial, sans-serif">Alcohol withdrawal syndrome</span></strong></p>
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<p><strong><span style="font-family: Arial, sans-serif">Alcohol withdrawal syndrome</span></strong></p>

Alcohol withdrawal syndrome

  • Alcohol craving

  • Irritability, tremor, nausea

  • Sleep disturbances 

  • High blood pressure, rapid heartbeat

  • Delirium tremors (DTs) in extreme cases

  • Agitation, confusion, hallucinations, nausea, diarrhea 

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Barbiturates/Benzodiazepines

  • All are retrieving the same symptoms like anxiety, sleep disorders, and seizures. 

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<p>Genes and Alcoholism </p>
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<p>Genes and Alcoholism </p>

Genes and Alcoholism

Two different studies found 40-60% of genetic contributions; these studies compared alcoholism rates in fraternal twins related in separate foster homes with alcoholism rates in identical twins reared in separate foster homes. Alcoholism was 3-5 times more likely to show up in identical twin pairs than in fraternal twin pairs. 

  • (last lecture): genetic predisposition sets you up to drink when you are stress which leads to addiction 

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Genetics of Alcoholism

  • Endophenotypes( a type of characteristic that have an origin that have multiple genes involved, combination of genes that contributes developing an alcohol addiction) link alcoholism in families

  • You have same genes as your parents and they develop alcohol dependence and addition and you have a genetic predisposition

  • Polymorphism (mutations below) 

  • ADH

  • ALDH

  • D2 and D4 receptors

Gives rise that you may develop alcohol addiction based on genetics 

  •  GABAA subunits 

  • 𝞪2 and 4, 𝛃1 and 𝛄1

  • Mutations here, have been show rise in alcohol addiction

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<p>Alcohol Toxicity </p>
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<p>Alcohol Toxicity </p>

Alcohol Toxicity

L-T is cirrhosis of liver and terminal death. 

  • Wernicke’s encephalopathy is a vitamin B1 (treated with) (thiamine) deficiency that occurs when cirrhosis 

  • confusion , ataxia and visual disturbances are symptoms. Reversible with vitamin B1 administration

  • Alcohol can also have a direct cytotoxic effect on the brain: loss of brain tissue (Korsakoff’s psychosis), this requires large amounts of alcohol intake for long periods of time. Irreversible loss of neurons

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Treatment for alcoholism

Disulfiram inhibits aldehyde dehydrogenase (block this enzyme so you have an increase of acetaldehyde) once you start giving this, people hate it because they get very sick.

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Naltrexone for treatment of alcoholism

  • naltrexone= opioid receptor antagonist 

  • Naltrexone is an opioid receptor antagonist that has been used as an adjunct to behavioral therapies. Naltrexone blocks opiate receptors that may be involved in the activation of dopamine neurons (stop reward system) in the VTA. This may reduce the reinforcing properties of alcohol. (extreme case) (beta-endorphin signals and shuts off gaba in vta, dopamine on, you have a reward system, having gaba on, shuts dopamine off and reward system is off)  

  • acamprosate= NMDA receptor antagonist   

  • Acamprostate blocks NMDA receptors. If these receptors are upregulated in alcoholics, inhibition of these receptors might relieve some of the anxiety associated with ethanol withdrawal and abstinence. (block withdrawal systems and long term treatment until NMDA receptors goes down) 

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<p>Alcohol dopamine pathway</p>
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<p>Alcohol dopamine pathway</p>

Alcohol dopamine pathway

VTA projection neuron into NA and PFC (dopamine neuron) but gaba took over. If gaba is activated, dopamine is inhibited (vice versa). When you are using alcohol, you will have one of the rxns, if there beta-endorphin, inhibits gaba and activates dopamine to get reward. Prolonged alcohol use releases endorphins. 


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<p>GABA(A) receptors </p>
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<p>GABA(A) receptors </p>

GABA(A) receptors

alpha1-alpha6

beta1-beta3

gamma1-gamma3

  • Penamere of the first stuff (know the percentages) Gaba binds between alpha and beta subunits. Baribiates bind with alcohol (ligand gated channels, allow influx of chlorine) 

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<p><strong><span style="font-family: Arial, sans-serif">Pentobarbital (PB) increases</span></strong></p>
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<p><strong><span style="font-family: Arial, sans-serif">Pentobarbital (PB) increases</span></strong></p>

Pentobarbital (PB) increases

Gaba causes an increase in negativity, hyperpolarizes the cell, gaba with alcohol does the same thing. Gaba with barbiturates have a maximum hyperpolarize with an influx of chloride ions

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<p>Barbiturates increase GABA(A) channel open time</p>
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<p>Barbiturates increase GABA(A) channel open time</p>

Barbiturates increase GABA(A) channel open time

<p>Barbiturates increase GABA(A) channel open time</p>
  • Each of these recordings came from the electrode of the cell. The dips (wells) that is live recording of a hyperpolarization with GABA. this is how GABA bind to GABA (A) receptors. You see more drastic dips with barbiturates because there is more hyperpolarization and it occurs for a longer period of time 

<ul><li><p><strong><span style="font-family: Arial, sans-serif">Each of these recordings came from the electrode of the cell. The dips (wells) that is live recording of a hyperpolarization with GABA. this is how GABA bind to GABA (A) receptors. You see more drastic dips with barbiturates because there is more hyperpolarization and it occurs for a longer period of time&nbsp;</span></strong></p></li><li><p></p></li></ul>
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<p><strong><span style="font-family: Arial, sans-serif">Cross Tolerance between barbiturates and alcohol</span></strong></p><p><br></p>
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<p><strong><span style="font-family: Arial, sans-serif">Cross Tolerance between barbiturates and alcohol</span></strong></p><p><br></p>

Cross Tolerance between barbiturates and alcohol


  • Alcohol tolerant need a lot more of barbiturates to get the anxiolytic effect. Alcohol naive needs less barbiturate since they are both working on the same receptor 

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Benzodiazepines

  • Xanax has better TI, and doesn't produce a narcotic effect. They decrease anxiety 

Chlordiazepoxide (Librium) was synthesized in the 1950’s as a sedative-hypnotic and anticonvulsant the most prescribed drugs in the world through the 1970’s-1980’s


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Atypical benzodiazepines

  • “Atypical” benzodiazepine receptor agonists such as zolpidem (ambien) and eszopiclone (Lunesta) have been developed and these are used as hypnotic drugs to treat sleep disorders

  • Benzodiazepines (alprazolam) are used to treat sleep disorders, epilepsy and anxiety. They are also used as sedatives and anesthetics for some surgical procedures (conscious sedation). They produce short lived sedation and amnesia

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<p><span style="font-family: Arial, sans-serif">𝛄-hydroxybutyrate (GHB) (GABA metabolism)&nbsp;</span></p><p></p>
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<p><span style="font-family: Arial, sans-serif">𝛄-hydroxybutyrate (GHB) (GABA metabolism)&nbsp;</span></p><p></p>

𝛄-hydroxybutyrate (GHB) (GABA metabolism) 

  • GHB is anxiolytic similar to alcohol and benzodiazepines (it basically is taking a lot more alcohol than just drinking alcohol by itself) 

  • Short half life (½ hour) (short pathway)

  • Synergistic effects with alcohol and produces amnesia at high doses.

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Propofol (Michael Jackson)

  • Drug of choice for induction of anesthesia and for short term surgical anesthesia. It is a powerful sedative and amnesia inducing drug.

  • Very safe and short acting and there is reduced “hangover” or drowsiness after use 

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Tests for innate anxiety  

Studies of the anxiolytic properties of a drug are difficult. Good predictive tests are important because it is desirable to separate the various effects of benzodiazepines in order to produce drugs that will produce only one type of effect. Two types of anxiety or fear responses: innate anxiety and conditioned anxiety. 

Innate anxiety: is shared by all (fear of open spaces in rodents). Conditioned anxiety is learned.

Tests for these types of anxiety conditions can be used to predict anxiolytic properties of drugs like the benzodiazepines

  • Elevated plus maze and novelty suppressed feeding are tests of innate anxiety in rats and mice 

  •  Benzodiazepines are anti-anxiety drugs

  • Decrease the latency to feeding in the novelty suppressed feeding assay

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<p><strong><span style="font-family: Arial, sans-serif">Anxiolytic effects of diazepam in the elevated plus maze</span></strong></p>
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<p><strong><span style="font-family: Arial, sans-serif">Anxiolytic effects of diazepam in the elevated plus maze</span></strong></p>

Anxiolytic effects of diazepam in the elevated plus maze

  • No treatment, mouse stays mostly in closed arms because it is scared. Adding a drugs, it will spend equal time of going in and outside the arm

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<p>Novelty Suppressed Feeding </p>
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<p>Novelty Suppressed Feeding </p>

Novelty Suppressed Feeding

A minute and half to get food. With drugs takes 10-20 seconds to get food

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<p>GABA(A) Receptors </p>
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<p>GABA(A) Receptors </p>

GABA(A) Receptors

  • BDZs bind to between  alpha and beta subunits , gaba bind to alpha and beta 

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Sedation vs Anxiolysis

The amygdala plays a central role in neuronal circuits responsible for anxiety 

  • a2b2g2 subunits found in highest concentration in the amygdala 

  • a1 subunits found in other brain areas

  • There are efforts to develop benzodiazepines like drugs that are selective for a2 subunit containing

  • GABAA receptors. These would reduce anxiety without causing drowsiness. This would be a benefit to the patient. 

  • Red and yellow, a lot of expressive of the subunit, blue and purple less expressive

  • Anterior amygdala see a lot of alpha 1

  • Anxiolysis alpha 2- specific to hit anxiety and avoid areas that causes seizures and anxiety 

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Endocannabinoid System

  • CB1: in CNS 

  • CB2: in PNS

  • Where different receptors are located and expressed (dots showed in image) (don’t memorize the list, know it in molecular level)


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<p><strong><span style="font-family: Arial, sans-serif">Two types of cannabinoid receptor&nbsp;</span></strong></p><p></p>
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<p><strong><span style="font-family: Arial, sans-serif">Two types of cannabinoid receptor&nbsp;</span></strong></p><p></p>

Two types of cannabinoid receptor 

  • Cannabinoid (CB) receptors are G-protein coupled receptors.

  • CB1 receptors are neuronal receptors (in CNS)

  • CB2 receptors are expressed by immune cell and microglial cells (macrophage type cells in the brain)

  • Both are coupled gcouple i and inhibit adenylyl cyclase and cyclic amp. Another layer of control is we increase a flow of K+ through gert channels and blocks calcium channels.

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<p>Signaling Pathways linked to cannabinoid receptors</p>
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<p>Signaling Pathways linked to cannabinoid receptors</p>

Signaling Pathways linked to cannabinoid receptors

-yellow bar is the membrane. Both receptors are embedded in the membrane. GalphaI we have a decrease in cyclic amp. K+ channels (activates) and ca2+(inhibits_ 


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Endocannabinoids

  • AEA–Agonists at CB1 and CB2 receptors (High affinity for CB1)

  • 2-AG–Agonist at CB1 & CB2

  • Come from arachidonic acid and forms anandamide, AEA and 2-Ag( don't know structures)

  • Both agonists for CB1 and CB2

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Exogenous Cannabinoids 

Rimonabant CB1 inverse agonist (2006 marketed by Sanofi)

All these signals in our bodies based on how much we ingest. Not tested

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Planted-Derived Cannabinoid Compounds

  • Cannabinoids are the group of more than 60 plant-derived compounds from Cannabis sativa

  • The primary psychoactive congener in marijuana is 9-tetrahydrocannabinol or 9-THC

  • Other plant derived cannabinoids are CBD little psychoactivity (cannabidiol) and CBN (cannabinol) 

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Synthetic cannabinoids (K2, Spice)


  • In the 70s is when they started to synthesize . spice- used as a spice on different herbs and can be smoked to get the effect (the two on the left) all can be K2. all produced in the lab by CB1 and have high potency and efficacy than THC.

  • Spice molecule relates to opiate receptor 

  • Can be used a surgical molecule for THC

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<p>CB1 VS CB2</p>
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<p>CB1 VS CB2</p>

CB1 VS CB2

  • Circles represent expression

  • Orange and yellow triangles are endogenous receptors and bind to CB1 and have high potency in CB1. 

  • AEA and 2AG have same level of potency in CB2

  • THC has higher potency in CB1

  • THC don;t have good potency in CB2

  • Receptors are presynaptic terminal, signals CB1and it prevents release of NTS in that nerve. Inhibition of ca2+ pulse because NT can’t be release. 

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<p><span style="font-family: Arial, sans-serif">Site of 9 - THC in the reward pathway&nbsp;</span></p><p><br></p>
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<p><span style="font-family: Arial, sans-serif">Site of 9 - THC in the reward pathway&nbsp;</span></p><p><br></p>

Site of 9 - THC in the reward pathway 


  • Ingesting THC creates a high because we are signaling dopamine. In VTA, GABA and projection neuron of DA from nucleus accumbens and it synapses back to DA. 

  • THC prevents binding CB1 and inactivating GABA and allows DA to be turned on and it turns on DA and glutamate neurons. And continue to open DA and circuit will be active which leads to addiction when glutamate is activated 


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<p><br></p><p><strong><span style="font-family: Arial, sans-serif">Pharmacokinetics: Routes of Administration</span></strong></p><p><br></p>
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<p><br></p><p><strong><span style="font-family: Arial, sans-serif">Pharmacokinetics: Routes of Administration</span></strong></p><p><br></p>


Pharmacokinetics: Routes of Administration


  • Ingest by injection, smoke, or eating. 

  • Level of euphoria experienced from ingesting weed. Intravenous or smoked has the shortest latency and highest blood concentration. Slowest way is through eating it and it has to go through ADME. 

  • Smoking and IV has shortest latency and oral has the longest latency 

  • Don’t have the same withdrawal effects like you would with opiates and alcohol.

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<p><strong><span style="font-family: Arial, sans-serif">Pharmacokinetics: Metabolism of 9-THC</span></strong></p><p></p>
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<p><strong><span style="font-family: Arial, sans-serif">Pharmacokinetics: Metabolism of 9-THC</span></strong></p><p></p>

Pharmacokinetics: Metabolism of 9-THC

  • Blood, urine and saliva tests can detect 9 -THC and its metabolites for as long as 1 month after the last use in heavy marijuana users. 5ng/mL is the intoxication threshold in Colorado.

  • 2 steps for 1st pass and carry by CYP2C9

  • 2nd pass of THC by glucuronyl transferase metabolism 

  •  9 -THC is psychoactive molecule and activates in largest molecule and shortest latency when smokes and quickie metabolize by 11-OH-THC (less signaling since it breaks down so quickly) and THC-COOH (most signaling in the body and sticks the longest). Little dash ends 1st pass of metabolism. In circulation for the longest time. 

  •  9 -THC has highest potency 

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Physiologic Responses to 9 THC Activity In the Brain know physiological response

  • VTA-NAcc: Reward Pathway 

  • Ventral tegmental area (VTA) Nucleus accumbens: reward pathway (mesolimbic dopamine system).

  • NTS: Nausea relief

  • Nucleus tractus solitarius- afferent input from the GI tract. Nausea and vomiting

  • Hypothalamus: Appetite stimulation

  • Hippocampus: memory

  • Amygdala: anxiety and wakefulness

  • Nucleus of meynert-cerebral cortex:cognition 

  • Periaqueductal gray: Pain Pathway 

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<p><strong><span style="font-family: Arial, sans-serif">9-THC in the Reward Pathway</span></strong></p>
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<p><strong><span style="font-family: Arial, sans-serif">9-THC in the Reward Pathway</span></strong></p>

9-THC in the Reward Pathway

  • Agonist at CB receptors 

  • Turns off-lost of  9 -THC in circulation

  • On (from above) 

  • Concentration goes up with potency

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<p><strong><span style="font-family: Arial, sans-serif">Endocannabinoids are retrograde neurotransmitters</span></strong></p>
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<p><strong><span style="font-family: Arial, sans-serif">Endocannabinoids are retrograde neurotransmitters</span></strong></p>

Endocannabinoids are retrograde neurotransmitters

  • Right half- signaling on presynaptic membrane and how release of NT happens. 


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9 -THC Suppresses Chemotherapy Nausea and Vomiting

  • 9-THC is a powerful antiemetic drug. Blocks chemotherapy induced nausea and vomiting. Chemotherapy drugs damage rapidly dividing cells including healthy cells including healthy cells in the GI mucosa.

  • Enterochromaffin cells in the GI mucosa are damaged by chemo drugs and these cells release 5-HT which stimulates vagal nerve fibers which terminate in the nucleus tractus solitarius (NTS) in the brain stem.

  • NTS stimulates nerves in the chemoreceptor trigger zone (CTZ) which stimulates neurons in the vomiting center to cause vomiting.

  • THC acts at inhibitory CB1 receptors on the vagal nerve terminal to inhibit glutamate release. This inhibits the vagal signal causing vomiting  

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<p>Presynaptic Vagal Nerve Terminal in the NTS</p>
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<p>Presynaptic Vagal Nerve Terminal in the NTS</p>

Presynaptic Vagal Nerve Terminal in the NTS

  • Brain stem, NTS connecting to CTZ where stimulus for nausea originates and goes through the  body for vomiting. Dominated serotonin receptors in enterochromaffin cell

  • THC signals through CB1 prevents nausea and vomiting.

NTS contains the vomiter cell and stimulate cells in stomach and NTS stimulates CTZ that cause vomit and nausea. THC binds CB1 prevents glutamate which reduce vomit and nausea


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Hypothalamic Regulation of Feeding Behavior

  • Cannabinoids act in the hypothalamus to stimulate appetite.

  • The arcuate and lateral hypothalamic nuclei of the hypothalamus control appetite and feeding behavior 


  • Activation of the lateral hypothalamus(LH) stimulates appetite and feeding. Inhibition of the LH suppresses appetite and feeding 

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VTA with cannabis

  • 3rd ventricle (dotted red)- arcuate nucleus. When you eat, stomach signals to brain and you are not hunger, arcuate nucleus will allow you to not eat. Having THC, inhibits from arcuate nucleus and it tell us to eat (munchies ) even when you are not hungry

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<p><strong><span style="font-family: Arial, sans-serif">CB receptors in pain and inflammation pathways</span></strong></p>
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<p><strong><span style="font-family: Arial, sans-serif">CB receptors in pain and inflammation pathways</span></strong></p>

CB receptors in pain and inflammation pathways

  • Macrophages are first responder inflammatory cells

  • Microglial are nervous system macrophages 

  • Inflammatory substances released from microglia and macrophages sensitize sensory nerve endings

  • THC acting on CB2 receptors inhibits macrophages and microglia

  • 9-THC stimulates neurons in the PAG which then stimulates 5-HT neurons in the raphe nucleus. These 5-HT neurons sends axons down the spinal cord to the dorsal horn where peripheral sensory neurons synapse with 2nd order sensory neurons. The 2nd order sensory neurons project up the spinal cord to the thalamus carrying pain signals.

  • The nerve terminals of peripheral sensory nerves in the dorsal spinal cord also have CB1 receptors. 9-THC acts on these receptors causing inhibition of Ca2+ channels and this prevents neurotransmitter release. Therefore, there is no signal coming from the peripheral sensory neurons to the 2nd order neuron. CB1 receptors are also on the peripheral nerve terminals (in tissues) which detect painful stimuli . 9-THC acts on these nerve terminals to inhibit them as well

  • CB2 receptors are expressed by immune cells such as macrophages and microglia. These cells become active at sites of tissue injury and they release substances that sensitize sensory nerve endings. This is part of the pain response at sites of tissue injury and inflammation

  • 9-THC acting at CB2 receptors inhibits macrophages and microglia from releasing inflammatory substances. This is part of the analgesic effects of 9-THC.


Brain to spinal cord (1st order)  and we have a sensory nerve, the red nerve (2nd order neuron). We get stimulus from sensory nerve (pain) into spinal column for sensory neuron on to 2nd oder neuron and will communicate to brain we have pain. 

Synapses onto 2nd order neuron, CB1 has its receptor in presynaptic bever, THC on bard prevents the transmission (ex:glutamate)) and block this pathway to the peripheral.

2: end of sensory nerve, these dendirtes of CB1, THC can signal there and shutting off pain closer to sensory and 2nd order neuron. 

CB2 receptors (periphery) micorgailia- inflammaoty response. THC acting on CB2 will blunt that to not feel pain and blocks inflammatory response. 


Singaling through CB2 will desynthrsize this 


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<p><strong><span style="font-family: Arial, sans-serif">Signaling pathways linked to cannabinoid receptors&nbsp;</span></strong></p><p><br></p>
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<p><strong><span style="font-family: Arial, sans-serif">Signaling pathways linked to cannabinoid receptors&nbsp;</span></strong></p><p><br></p>

Signaling pathways linked to cannabinoid receptors 


Both GCPR once an agonist, the alpha will dissociate and g alpha i inhibits the adenyl cyclase and cAMP. CB1 activates potassium and inhibits calcium. 


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<p><strong><span style="font-family: Arial, sans-serif">9-THC induced programmed cell death (apoptosis) in T-cells of the immune system (first thing)&nbsp;</span></strong></p><p><br></p>
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<p><strong><span style="font-family: Arial, sans-serif">9-THC induced programmed cell death (apoptosis) in T-cells of the immune system (first thing)&nbsp;</span></strong></p><p><br></p>

9-THC induced programmed cell death (apoptosis) in T-cells of the immune system (first thing) 


  • T-cells facilitate immune system activation

  • T-helper cells, Natural Killer cells, memory cells, B-cells) 

  • Due to CB2 signaling at THC (caused above)

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Cannabinoid Pathway

  • Inhibition cAMP and decrease signaling cascade and pro-inflammatory gene expression. Decrease immune response

<ul><li><p><span style="font-family: Arial, sans-serif">Inhibition cAMP and decrease signaling cascade and pro-inflammatory gene expression. Decrease immune response</span></p></li></ul>
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Crohn’s Disease 


  • Chronic inflammation of the last part of the small intestine (ileum) and first part of the colon.

  • Caused by inappropriate immune response to normal bacteria living in the gut (microbiota)

  • Can be hereditary 

  • Chronic diarrhea, intestinal bleeding, abdominal pain, weight loss, fatigue 

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<p><strong><span style="font-family: Arial, sans-serif">Smoking cannabis improves Crohn’s disease activity index (CDAI)</span></strong></p><p><br></p>
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<p><strong><span style="font-family: Arial, sans-serif">Smoking cannabis improves Crohn’s disease activity index (CDAI)</span></strong></p><p><br></p>

Smoking cannabis improves Crohn’s disease activity index (CDAI)


  • Lower the score, the better the person is doing. 

  • Weekly treatments and then they did not get anything and got treatment again.

  • The drop in CBAI in people getting THC. THIs Improves condition with chrons. 

  • (-) 1(no effect) 7(very effective)

  • (+) 1 (stasified) 7(not statsifed)

  • Effect shows in positive and got less pain, appetite

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Cannabidiol enriched marijuana for treatment of childhood epilepsy \


  • 19 patients and ages between 2-16

  • Seizures before CBD, people had over 100 seizures to 8-10 a day.

  • Reduction of seizures once they receive CBD treatment.

  • 3/19 did not get the effect of CBD helped. 25-80% decrease of seizures in the 16 patients.

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Cannabidiol in Dravet’s Syndrome

(placebo controlled/double blind) 


  • 40% difference in cannabidiol and placebo. CBD helps this condition.

  • Side effects: Sommolence, lethargy, decreased appetite, vomiting 


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Multiple sclerosis


  • Multiple sclerosis is an autoimmune disease in which the immune system becomes dysregulated.

  • Autoimmunity causes demyelination of motor axons and inflammation.

  • MS symptoms includes fatigue, muscle weakness, numbness in arms and legs, muscle spasticity and difficulty walking.

Anti-inflammatory effects of 9-THC/cannabidiol relieve symptoms.

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Sativex (9-THC/CBD; oral mucosal spray)


People taking sativex vs placebo reported less muscle spasms . more on sativex, less muscle spasms that happens with MS.

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Anandamide release from CA1 neurons…

Anandamide release from CA1 neuron inhibits GABA interneurons

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<p><strong><span style="font-family: Arial, sans-serif">9-THC impairs spatial learning and memory in mice</span></strong></p>
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<p><strong><span style="font-family: Arial, sans-serif">9-THC impairs spatial learning and memory in mice</span></strong></p>

9-THC impairs spatial learning and memory in mice

  • Black square- stage

  • White liquid and the mouse can stand on platform. Researchers removes platform and the Mouse going to swim to find platform. 

  • Day 5: they swim around but know where the platform is.

  • With THC, they have a harder time finding it. Both groups found a platform on day 5 then day 1.

  • Not statistically significant but for adolescents it is. 

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9-THC suppresses Eeg Gamma oscillations (fastest brain waves)


  • Associated with learning and memory formation.

  • Couldn't control the users that were taking on like alcohol.

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<p>Electroencephalogram (EEG)</p>
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<p>Electroencephalogram (EEG)</p>

Electroencephalogram (EEG)

  • Study shows when you have people treated with cannabis and control, GAMMA oscillations decrease with people with cannabis. This has a larger effect with the younger the people go. Younger you go down, the worse you are for your brain to handle. 

  • THC can be a problem with young brain developing for learning and memory 

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<p><strong><span style="font-family: Arial, sans-serif">Medical Marijuana- Drug Scheduling</span></strong></p><p><br></p>
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<p><strong><span style="font-family: Arial, sans-serif">Medical Marijuana- Drug Scheduling</span></strong></p><p><br></p>

Medical Marijuana- Drug Scheduling


  • Schedule 1-5 determined by:

  • Abuse potential

  • Medial use


  • Criteria for drug to be a medicine

  • Chemistry must be known and reproducible 

  • Adequate safety studies

  • Well-controlled studies proving efficacy 

  • Accepted by qualified experts

  • Evidence widely available 

    Tetrahydrocannabinol and marijuana are Sch1

  • Dronabinol Sch3

Trail 1- highest abuse, no medical use

2: high abuse, some accepted medical use


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Cannabinoid Pharmaceuticals- Dronabinol (Marinol)

  • Synthetic 9-THC in sesame oil (droplet)

  • Indications: attenuation of nausea associated with cancer chemotherapeutics and appetite stimulation 

  • Oral administration

  • Marketed in US


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Cannabinoid Pharmaceuticals- Nabilone (Cesamet)

  • Synthetic analog of 9-THC (capsule)

  • Indications; attenuation of nausea associated with cancer chemotherapeutics and appetite stimulation 

  • Oral administration

  • Marketed in Canada  

oral administration
– results in slow onset
– lower bioavailability than inhalation – difficulty with compliance if severe vomiting

• neither approved drug has found much utility because of the narrow window between effective dose and unwanted side effects 


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Cannabinoid Pharmaceuticals-Rimonabant (Acomplia in EU, Zimulti in US)

  • CB1 receptor antagonist (synthesized compound)

  • Indications: adjuvant to diet and exercise for treatment of obesity

  • Oral administration

  • FDA advisors rejected approval because of suicidal tendencies; causes powerful nausea

  • Discontinued in EU in late 2008) 

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Cannabinoid Pharmaceuticals- Sativex

  • Combination of THC and CBD

  • Anti-spasticity drug for MS patients

  • Efficacious for preventing muscle spasms, pain

  • Marketed in Canada, Europe, S.Pacific

  • First therapeutic cannabinoid available in a form other than capsule (oromucosal spray) 

  • Other indications for Sativex include neuropathic pain that may or may not be associated with MS or cancer

  • CNS side effects are lowered relative to a “9-THC only” preparation 

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