Micro Lab Quiz 1

why do you wear a lab coat?

Protects your clothing/body from contamination and exposure

when do you wash your hands?

Before AND after lab work

when do you disinfect the lab bench

Before AND after lab work

How should cultures be treated?

As potential pathogens

pathogens

disease causing agents

aseptic technique

method used to make the environment, the worker, and the patient as germ-free as possible

What technique should be used when handling cultures?

aseptic technique

What do you do with Sharpie writing on tubes before disposal?

Remove it with ethanol

What do you do with labeling tape?

Remove it before disposal

What do you do with broken glass?

Broom/dustpan; don't touch with hands.

What is total magnification with 40× objective and 10× ocular?

400x magnification

objective lens

lens on the microscope that is closest to the specimen and provides the magnification.

X on objective lenses

4×, 10×, 40×, and 100×

Coarse focus

Knob that allows you to make major focusing adjustments. (large knob)

Fine focus

Knob that allows you to make precise focusing adjustments. (small knob)

What do you use to clean microscope lenses?

Lens paper

What order do you clean objectives?

→ 4× → 10× → 40× → 100×

What lens is cleaned last?

→ 100× oil immersion.

Why lower the coverslip slowly?

To avoid air bubbles.

What magnification do you start with when viewing a wet mount?

4x- Low power.

wet mount

liquid specimen placed on a slide and covered with a coverslip for viewing under a microscope

incubation

Keeping microbes under suitable conditions so they can grow

contaminant

An unwanted microorganism introduced into a sample/culture/ env.

colony

visible mass of microorganisms growing on solid media (agar plate)

correct way to label

Dispose of plates

designated biohazard disposal area/bin

dispose of tubes

remove labeling (sharpie removed w ethanol), discard pile at back of room

how to get total magnification

ocular lens x objective lens

Inoculating loop sterilization

electric incinerator

5-7 seconds only.

prokaryote

cell that does not have a nucleus

eukaryote

cell contains a nucleus

main difference between prokaryotes and eukaryotes?

Pro: lack a nucleus

Euk: have a nucleus

Bacillus subtilis, E. Coli, Tolypothrix

Prokaryote; it is a bacterium.

Saccharomyces cerevisiae, Chlamydomonas, Spirogyra, protozoa

Eukaryote, yeast

colony morphology

Physical characteristics of bacterial colonies.

colony shape/form

overall shape of a colony when viewed from above

colony shapes

Circular, irregular, and rhizoid

What does a rhizoid colony look like?

Root-like or branching

What is the margin of a colony?

edge of colony

What is an entire margin?

A smooth, even edge.

colony elevation

The shape/height of the colony when viewed from the SIDE.