EXP 1: IDENTIFICATION OF AMINO ACIDS BY PAPER CHROMATOGRAPHY

Paper Chromatography

A method of separating and identifying amino acids

polar liquid phase, cellulose, eluting solvent

Paper chromatography involves the distribution of the solute between the ________ __________ _________, whis is strongly adsorbed on the _________ fibers of the paper, and the __________ _________.

up

The mobile phase moves ? the paper.

1. pH
2. Temperature
3. Concentration of the solvent
4. Time of chromatography

The location of amino acids depends on several factors namely:

0.2% Ninhydrin solution

What is sprayed to determine the position of the amino acids?

Butanol
Acetic Acid
Distilled Water

What reagents are mixed inside the developing chamber?

4:5:1

Ratio of reagents in the developing chamber

0.5% Lysine
0.5% Glutamic Acid
0.55 MSG
Canned food

4 substances checked for amino acids

(Distance amino acid) / (Distance of solvent)

Rf value formula

Rf value

a ratio that compares the distance a substance travels to the distance a solvent travels in chromatography