Cell Theory
All organisms are made of cells, basic unit of life, all cells come from preexisting cells
To be labeled as living there needs to be
reproduction, response to stimuli, growth/development, homeostasis, use of energy
Microscopes where founded by who
Robert Hooke in 1665
What are some of the different types of a microscope
light microscope, transmition electron microscope(TEM), scanning electron microscope(SEM)
Light microscopes are used for
living cells
electron microscopes are used for
dead cells
TEM
used to study internal parts of cells
SEM
lets us see the surface structure of cells
Cell fractionation
separate organelles from cells, variable density of organelles-ultacentrifuge
centrifuge
technique to separate
All cells have
plasma membrane, cytosol, chromosomes, ribosomes
cytosol
semi-fluid substance within the membrane
Prokaryotic
DNA in nucleoid region, w/o membrane separating it from the rest of the cell
Eukaryotic
chromosomes in nucleus, membrane enclosed organells, more complex, generally bigger, cell specialization
Surface are to volume ratio
as cells get bigger its volume increases faster than its surface area, smaller objects have greater ration of surface are to volume, SMALLER cells diffuse faster than LARGER cells
nuclear envolope
pores
Nuclear lamina
a structure near the inner nuclear membrane and the peripheral chromatin
nucleolus
inside the nucleus, makes ribosome components
Endomembrane system
nuclear envelope, endoplasmic reticulum, golgi apparatus, lysosomes, vesicles, vaculoles, plasma membrane
Golgi has two sides
cis side- receiving side and trans side- shipping side
Autophagy
lysosomes breaking down damaged organelles
Endosymbiosis
theory that mitochondria and chloroplasts were once prokaryotic that were consumed by eukaryotes and became endosymbiotic
mitochondria
folds in inner membrane increase the surface, no mitochondria=not much energy
Chloroplast
plastid that contains chlorophyll
Peroxisome
contains enzymes that remove hydrogen from various substrates and makes hydrogen peroxide. Prevents cells from harmful toxic substances
Cytoskeleton
functions in structural support for the cell network for fibers that organizes structures and activities within the cell-made of proteins sometimes complex carbohydrates
Cilia+flagella
motile appendages containing microtubules
protein tubes
microtubules
cilia
short usually numerous, hairlike structure
flagella
long, fewer, whip-like structure
Cell Wall
found in plants, bacteria, and fungi
plants
composed of cellulose
bacteria
composed of peptidoglycan
fungi
composed of chitin
cell junctions
neighboring cells often adhere, interact, and communicate via sites of direct contact
plasma membrane
edge of living/nonliving, structure determines function, selectively permiable
amphipathic
has both a hydrophobic and hydrophilic region
membranes are bifacial
they have distinct inside and outside faces
proteins on the outside of membranes are unstable
not soluble in water they are amphipathic
integral proteins
at least partially into hydrophobic areas
peripherial proteins
not in hydrophobic region
cholosterol
lipid, steroid
carbohydrate
only on extracellular side(outside)
glycoprotein and glycolipids
cells are structured by what
matrixs(nonliving stuff)
temp the membrane solidifies at
depends on its compostition
cholesterol acts as a
fluidity buffer which resists changes in the membrane due to temp changes
some cells can alter lipid composition of membraneas
cold adapted organisms and prevents membranes from solidifying during winter
the hydrophobic regions of an intergral protein
consists of one or more stretches of nonpolar amino acids, often coiled into alpha helices
six major functions of proteins in cells
transport, enzyme activity, signal transduction, cell-cell recognition, inter cellular joining, attachment to cytoskeleton and extracellular matrix(ECM)
bifacial
two lipid layers differ in composition, anything w/ a membrane is bifacial
hydrophobic(nonpolar molecules)
such as hydrocarbons, can dissolve in the lipid by layer
polar molecules
sugars cant cross the membrane as easy
carrier proteins
bind to substances and physically move it across the membrane
aquaporins
allows entry of water, passing through single file through its central channel, doesnt bond
passive transport
is diffusion of a substance across a membrane w/ no energy invesment
diffusion
high to low
dynamic equalibrium
many molecules cross one way as they cross in one direction
osmosis
passive transport of water, diffusion of water across a selectively permeable membrane
tonicity
the ability of a surrounding solution to cause a cell to gain or lose water. Based on solute concentration and membrane permeability
hypotonic
solution w/ lower solute concentration compared to inside
isotonic
equal solute concentration on inside and outside
hypertonic
solution w/ greater solute concentration than whats inside
osmoregulation
the concentration of water balance, adaptation
globular proteins
doors in membrane, channel or carrier protein, channels to over specific molecules through cell membrane
ion channels
open or close in response to a stimulus(gated channels- passive transport, bonds to it to open")
Active transport
moves substances against concentration gradient
ion pumps
all cells have voltages across their membranes, electrogenic pumps, protein pumps
cotranspot
a single ATP powered pump that transports a specific solute can indirectly drive the active transport of several other solutes in a mechanism
bulk transport
using vesicles
exocytosis
exit large molecules
endocytosis
enter large molecules
ligand
message receptor, outside membrane
phagocytosis
cell eating
pinocytosis
cell drinking
receptor mediated endocytosis
responding to message