Hematology Laboratory Reference Values and Blood Composition

Reference values for RBC/WBC counts, differentials, indices, hemoglobin concentrations, and blood membrane composition based on clinical laboratory standards.

RBC Count (Male, Conventional Unit)

$$4.5-6.0\,\text{M/cu.mm}$$

RBC Count (Female, S.I. Unit)

$$4.0-5.5 \times 10^{12}/L$$

WBC Count (S.I. Unit)

$$5.0-10 \times 10^9/L$$

Normal Relative Value: PMN

$$57-65\%\,\text{or}\,0.57-0.65$$

Normal Relative Value: Lymphocytes

$$23-35\%\,\text{or}\,0.23-0.35$$

Normal Relative Value: Monocytes

$$4-8\%\,\text{or}\,0.04-0.08$$

Normal Relative Value: Eosinophil

$$2-5\%\,\text{or}\,0.02-0.05$$

Normal Relative Value: Basophils

$$0-1\%\,\text{or}\,0-0.01$$

Normal Absolute Value: PMN

$$2,850-6,500/\text{cu.mm}\,(2.85-6.5 \times 10^9/L)$$

Normal Absolute Value: Monocytes

$$200-800/\text{cu.mm}\,(0.2-0.8 \times 10^9/L)$$

Schilling Count (Stabs)

$$2-5\%$$

Reticulocyte Count (Adults, Conventional)

$$0.5-1.5\%$$

Reticulocyte Production Index (Inadequate erythropoietic response)

$$\text{RPI less than 2}$$

Reticulocyte Production Index (Adequate bone marrow response)

$$\text{RPI greater than 3}$$

Hemoglobin Concentration (Men, Conventional)

$$14-18\,\text{g/dL}$$

Hemoglobin Concentration (Newborns, S.I.)

$$165-215\,\text{g/L}$$

Hematocrit (Male)

$$40-54\%\,\text{or}\,0.40-0.54\,\text{L/L}$$

Arneth Count: Class I

Nucleus Unsegmented ($5\%$)

Arneth Count: Class III

$$3\,\text{Nucleus Lobes}\,(41\%)$$

Arneth Count: Class V

$5\,\text{Nucleus lobes}\,(2\%$)

MCV (S.I. Unit)

$$80-100\,\text{fL}$$

MCHC (Conventional Unit)

$$30-36\%$$

Erythrocyte Sedimentation Rate: Westgren Method (Male)

$$0-15\,\text{mm/hr.}$$

Erythrocyte Sedimentation Rate: Wintrobe Method (Female)

$$0-15\,\text{mm/hr.}$$

Blood Component: Plasma

$$55\%\,\text{of total blood}$$

Blood Component: Formed Elements

$$45\%\,\text{of total blood}$$

Blood Component: Buffy Coat

$$<1\%\,\text{of total blood}$$

RBC Membrane: Proteins

$$52\%$$

RBC Membrane: Lipids

$$40\%$$

Normal Hemoglobin: HbA1

$$90\%$$

Normal Hemoglobin: HbF

$$<2\%$$

Hemoglobin Derivative: Oxy-Hb

$$97\%\,(\text{red})$$

Hemoglobin Derivative: Deoxy-Hb

Cyanosis $>5\%\,(\text{purple})$

Hemoglobin Derivative: CO-Hb

$$0.16\%\,(\text{Cherry Red})$$

Hemoglobin Derivative: Sulph-Hb

High in cockroaches (green)

Osmotic Fragility Test (Sanfrod Method: Initial Hemolysis)

$$0.44\,\text{or}\,0.42\%$$

Osmotic Fragility Test (Dacie Method: Complete Hemolysis)

$$0.35-0.30\%$$