BCH210: Edman Degradation Enzymes

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Last updated 4:49 AM on 5/25/26
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4 Terms

1
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Trypsin

Cleaves after lysine (K) and arginine (R), when proline (P) is not present. 

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Chymotrypsin

Cleaves after large, hydrophobic –R groups

> tryptophan (W), tyrosine (Y), phenylalanine (F), leucine (L), methionine (M)

3
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Cyanogen bromide

Cleaves after M

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Carboxypeptidase A

Cleave the C-terminus end, unless in the presence of K, R, or P