Lab 4 Bacterial Morphology / staining

Why do we heat fix slides

Kill bacteria by denaturing protiens and allows organisms to be attached to the slide

What reagent does simple staining use

Crystal violet

What does simple staining allow you to see

Size Shape and Arrangement

How must you view simple stained bacteria

using the Oil-immersion power

What are Rod shapped bacteria called

Bacillus

What are oval shapped bacteria called

Coccus

What are bacteria found in clusters called

Staphylococcus

What is it called when bacteria are arranged in pairs

Diplo

What is it called when bacteria are in chains

Strepto

What bacteria is this

E. Coli, rods

What type of bacteria is this

Staphylococcus

What are the 4 reagents for a gram stain

Crystal violet, Iodine, Ethanol, Safranin

What does Iodine do in a gram stain

help the crystal violet bind inside the CW

What does the Ethanol do during a gram stain

it de-colorizes the gram negative bacteria

What is this gram stain showing

Gram negative E.Coli

Whats an example of bacteria with a capsule

Pasteurella multocida

Whats an example of bacteria with endospores

Clostridium sp. & Bacillus sp.

What host defenses do capsules allow bacteria to resist

lysozyme and phagocytosis

what is an acid fast positive

red/pink

what is an acid fast negative

blue

What does Acid fast positive cell have

Waxy cell wall and has PG

What does the waxy cell wall help with

shield against chemicals dehydration

What is this bacteria

M. Leprae