Enzyme Lab

This set of flashcards covers key concepts related to enzyme activity, specifically focusing on invertase, experimental methods, safety protocols, and testing procedures as outlined in the lab notes.

What is the purpose of the lab involving invertase?

To perform experiments illustrating the relationship between enzyme activity and four parameters: substrate amount, enzyme amount, pH, and temperature.

What enzyme will you be working with in the lab?

Invertase, which comes from the yeast Saccharomyces cerevisiae.

What does invertase catalyze?

The hydrolysis of sucrose into glucose and fructose.

How will enzyme activity be measured in this lab?

Using the Benedict's Test to detect the presence of simple reducing sugars.

What color is Benedict's solution before it reacts with sugars?

Blue.

What happens to Benedict's solution when it reacts with simple sugars?

It forms a different-colored precipitate, ranging from light green to brick red.

What type of sugars does Benedict's Test detect?

Simple reducing sugars (monosaccharides with free aldehyde groups).

What is the function of a burette in this lab?

To dispense precise volumes of liquid solutions.

What should be done if pH buffer or Benedict's solution comes into contact with the skin?

Flood generously with water.

Why is eye protection necessary in this lab?

Because you will be working with acids and bases.

What safety caution is advised when using water baths?

Be careful when working with high temperature water to avoid burns.