Biotechnology & Microscopy Notes

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6 Terms

1
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Which component is necessary for creating a recombinant plasmid during bacterial cloning?

restriction enzymes

2
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Which technique helps in locating specific sequences in DNA after electrophoresis?

Western Blotting W

3
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What is confocal laser scanning microscope?

see structures in thick samples using fluorescent tags

4
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What is the RNA world hypothesis?

RNA can catalyze chemical rxns like enzymes

RNA stores genetic info like DNA

Self-replicating molecules were precursors to life

5
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What 2 things do reverse transcriptase do?

used to make DNA from RNA

make complementary DNA from mRNA because they lack introns

6
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How does recombinant Dna work?

use restricted enzymes that cut DNA at restricted sites that create sticky ends and allow new DNA to bind. DNA ligase connects them. A vector is used to transfer foreign DNA