Venipuncture Procedure Steps

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Last updated 5:38 PM on 7/22/26
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22 Terms

1
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Step 1

Verify the requisition form for patient information and tests required.

2
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Step 2

Properly identify the patient.

  • Ask patient to state their full name and DOB

  • Ask patient to spell first and last name

  • Compare information to request form

  • If patient is wearing a band, it must match

3
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Step 3

Introduce yourself

Explain the procedure and secure patient consent

4
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Step 4

Wash hands and put gloves on

5
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Step 5

Do not label tubes before venipuncture

6
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Step 6

Gather equipment

7
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Step 7

  • Assess the appropriate venipuncture site

  • Apply tourniquet

8
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Step 8

Palpate vein

9
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Step 9

  • Clean area with alcohol swab

  • Allow disinfectant to AIR DRY

  • Also clean gloved fingers with alcohol

10
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Step 10

Assemble needle and tube holder then inspect needle for any damage

11
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Step 11

Use thumb to anchor vein BELOW venipuncture site

12
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Step 12

Make sure bevel is facing up and insert needle at 15-30 degree angle

13
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Step 13

Once needle is in vein, push tube into holder

14
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Step 14

Release tourniquet

15
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Step 15

Fill needed tubes

16
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Step 16

Hold gauze over puncture site and apply pressure immediately AFTER removing needle

17
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Step 17

Activate needles safety and dispose into sharps container

18
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Step 18

Label tubes

  • Patients full name

  • DOB

  • Date and time of collection

  • Tech’s initials

19
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Step 19

Place tubes in transport bag

20
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Step 20

Check puncture site to see if bleeding stopped

21
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Step 21

Clean and dispose of everything properly

22
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Step 22

Remove gloves one at a time turning them inside out

  • say goodbye to patient and let them know a physician will deliver their results