chemsitry weekly quiz 2

A breakdown product of hemoglobin is:

Bilirubin

Hemoglobin S can be separated from hemoglobin D by:

electrophoresis on a different medium and acidic pH

On electrophoresis at alkaline pH, which of the following is the slowest migrating hemoglobin?

HbC

Given that the controls are within range each day, what is a probable explanation for the abnormal result on day 4?

specimen had prolonged exposure to light

Urobilinogen is formed in the:

intestine

if the total bilirubin is 3.1 mg/dL (53.0 mol/L) and the conjugated bilirubin is 2.0 mg/aL (34.2 mol/L), the unconjugated bilirubin is:

1.1 mg/dL (18.8 umol/L)

Ten heparinized plasma samples are assayed for bilirubin by 1) Jendrassik-Grof method on analyzer 1, and by 2) the Evelyn-Malloy method on analyzer 2, in a method comparison study.

The results are all 10-20% higher from analyzer 2 most likely because:

alcohol reagents in method 2 cause precipitation of proteins and increased background turbidity

In the liver, bilirubin is converted to:

bilirubin diglucuronide

In which of the following disease states is conjugated bilirubin a major serum component?

biliary obstruction

Kernicterus is an abnormal accumulation of bilirubin in:

brain tissue

In which of the following conditions does decreased activity of glucuronyl transferase result in increased unconjugated bilirubin and kernicterus in neonates?

Crigler-Najjar syndrome

A stool specimen that appears black and tarlike should be tested for the presence of:

occult blood

In amniotic fluid, the procedure used to detect hemolytic disease of the newborn is:

measurement of absorbance at 450 nm

Hemoglobin S can be separated from hemoglobin D by which of the following methods?

citrate agar gel electrophoresis at pH 5.9

Before unconjugated bilirubin can react with Ehrlich diazo reagent, which of the following must be added

caffeine

The most widely used methods for bilirubin measurement are those based on the:

Jendrassik-Grof method

in the Evelyn-Malloy method for the determination of bilirubin, the reagent that is reacted with bilirubin to form a purple azobilirubin is:

diazotized sulfanilic acid

In the Jendrassik-Grof method for the determination of serum bilirubin concentration, quantitation is obtained by measuring the green color of:

azobilirubin

In the Jendrassik-Grof reaction for total bilirubin, alkaline tartrate is added to:

eliminate many spectrophotometric interferences

The laboratory results shown in this table are obtained on a patient suspected to have liver disease.

total bilirubin	9.5 mg/di
unconjugated bilirubin	8.5 mg/di
urine bilirubin	negative
urine urobilinogen	increased

The type of jaundice would be classified as:

Pre hepatic

The laboratory results shown in this table are obtained on a patient suspected to have liver disease.

total bilirubin	9.5 mg/dL (162 umol/L)
unconjugated bilirubin	1.5 mg/dL (26 umol/L)
urine bilirubin	positive
urine urobilinogen	decreased

The type of jaundice would be classified as:

post hepatic

A quantitative measurement of urobilinogen uses the following reagent:

Ehrlich

The most specific enzyme test for acute pancreatitis is:

Lipase

Which of the following enzymes are used in the diagnosis of acute pancreatitis?

amylase (AMS)

Which of the following enzymes catalyzes the conversion of starch to glucose and maltose?

amylase (AMS)

What enzyme is measured in the reaction of maltoheptaose-nitrophenol producing glucose fragments and 4-nitrophenol?

amylase

Aspartate aminotransferase (AST) is characteristically elevated in diseases of the:

Liver

Aminotransferase enzymes catalyze the:

exchange of amino and keto groups between alpha-amino and alpha-keto acids

Aspartate aminotransferase (AST) and alanine aminotransferase (ALT) are both elevated in which of the following diseases?

Viral hepatitis

a significant source of which of the following enzymes within RBCs should be considered during interpretation of and sources of interference in serum enzyme analysis?

AST

Which of the following clinical disorders is associated with the greatest elevation of lactate dehydrogenase isoenzyme 1?

Pernicious anemia