Micro 202L study Guide

Agar

What is used to solidify media?

Date, microorganism, initials

What information is included when labeling tubes and plates?

Smear

A suspension of cells dried onto a slide is called

Molds

Which one of the following microorganisms produce growth that is usually think, fuzzy, or furry?

Streak plate

Method used to obtain isolated colonies form a mixed culture of bacteria?

Budding

Yeast asexually produce by

Heat fixing

Before staining what must be done to your air dried bacterial smears so that the cells adhere to the slide?

Optical lens wipe

Which of the following items can be used to clean the objectives prior to putting your microscope away?

Bacillus, spiral, coccus

The three main bacterial cells shape include

1000x

When examining microbes using the oil immersion objective, total magnification is?

1. Place a loop full of water of the slide

2. Mix in bacteria

3. Dry

4. Heat fix

Order of steps for making a bacteria smear

Filtration

A safe method of sterilizing heat sensitive liquids such as vaccines and antibiotic solutions is?

Aseptic techniques

Methods used to avoid contamination

Surface disinfectant

_____ is not a sterilization method

True

Eukaryotic are more related to archaea than bacteria

A very high amount of salt (7.5% NaCl)

Mannitol salt agar is selective for member of the genus Staphylococcus because it contains

Beta-hemolysis

The ability to completely lose red blood cells is known as

Nutrient agar

Growth Medium

Nutrient agar with streptomycin

Selective

Mannitol salt agar

Selective and differential

Blood agar

Differential

Oxygen

Obligate aerobes require ____ to grow

Gram negative

When doing a gram stain, cells are decolorized by ethanol (thus making the counter stainable with safranin) are called____

Photoreactivation

Some microorganisms are able to enzymatically repair DNA damaged by ultraviolet radiation is they are exposed to strong visible light. This is called_____

Random mutations, overuse of antibiotics, unnecessary use

What helped create antibiotic resistance?

Purple

Gram positive with gram stain

Red

Gram positive With acid fast staining

Pink

Gram negative with gram stain

Blue

Gram negative with acid-fast straining

True

Staphylococcus aureus can be a pathogen in people, despite the fact that about 20-25% of the population are carriers of this microorganism.

Mesophiles

Which category of microbes would you expect best to grow on or in your body?

Staphylococcus aureus

This bacteria is gram positive, produces a yellow pigment, and is beta hemolytic

Tuberculosis

Even though it was developed in the 1880’s, the acid fast staining is still important because it is an aid in the diagnosis of

Needs oxygen

Obligate aerobe

Not growth with oxygen

Obligate anaerobe

Can do with or without oxygen

Facultative anaerobe

Differential

The gram stain and acid fast staining are considered ____ stains

Crystal violet

Gram stain Primary stain

Lugol’s iodine

Gram stain Mordant

Ethyl Alcohol

Gram stain Decolorizer

Safranin

Gram stain secondary stain

True

The longer the bacteria is exposed to UV light the greater the killing effect observed

High-lipid

Acid fast staining bacteria have what type of cell wall

To distinguish between acid fast and non acid fast bacteria

What is the purpose of acid fast staining?

Isotonic

Cells have no net movement of water in this type of solution (it does provide an excellent environment for bacterial growth)?

Mutation acquired antibiotic resistance

In exercise 10, we inoculated Serratia marcescens onto agar plates contain streptomycin, an antibiotic that normally kills this bacteria. However due to _____, a very small minority of the bacteria did survive and grow.