quiz 7 Restriction Enzymes and Gel Electrophoresis

Plasmids are commonly used as vectors for genetic cloning because they are…

a) small in size

b) Easy to manipulate

C) A&B

d) None of the above

Plasmids are typically _____and______ strands of DNA in the cytoplasm of bacterium

a) big, linear

b) small, linear

c) small, circular

d) big, circular

Plasmids are commonly used as vectors for genetic cloning because they are…

a) small in size

b) Easy to manipulate

C) A&B

d) None of the above

Plasmids are typically _____and______ strands of DNA in the cytoplasm of bacterium

a) big, linear

b) small, linear

c) small, circular

d) big, circular

what is the bacterial enzyme that cut double stranded DNA at specific sites unique to the enzyme?

a) Kinases

b) Bibrio harveyi

C) Restriction endonucleases

d) Plasmids

In gel electrophoresis, ____ is the stain used in the gel itself to allow us to see the glowing fragments under UV light, while ____ is the stain used in the samples to allow us to see them with the naked eye and to “sink” them through the buffer and into the wells

a) Ethidium Bromide; loading dye

b) loading Dye; Ladder

c) loading dye; Ethidium bromide

d) Buffer, ladder

what is the charge of DNA?

a) Positive

b) Negative

In gel electrophoresis to which charge does DNA move towards to?

a) Positive

b) negative

in the image on the right: what is the approximate size (in base pairs) of fragment #2

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Write FULLNAME of the Nuxleotide C and T

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somatic cells are ploid while gametice cells are ploid

??

True or False: Restriction sites are generally not palindromic sequences

?

True or False: Plasmids typically code for specific functions that are not essential to the life of the cell

?

True or False: Gel electrophoresis can separate fragments of DNA based on mass, size, shape, or charge

?

True or false: in DNA, C pairs with T

?

True ot false: All of the DNA in a cell makes up what is called a genome

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True or false: Bacteriophages are viruses that infect bacteria

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who is the monk that discovered the principles that govern heredity in the 1860’s?

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