Sugar in Honey Lab

Flashcards about the Sugar in Honey Lab

What is the initial step in preparing honey samples for sugar analysis?

Dilute the honey with distilled water and filter to remove particulates.

Which enzyme is utilized to quantify glucose levels in the honey sample?

Glucose oxidase, which specifically reacts with D-glucose.

Which reagent serves to identify all reducing sugars present in the sample?

Nelson's reagent, which reacts with reducing sugars to form a color change.

How is the color change quantified in both assay types?

Using a spectrophotometer to measure absorbance.

How is the fructose level estimated?

By subtracting the glucose concentration (from the enzyme assay) from the total reducing sugars (from Nelson's reagent assay).

Why isn't fructose measured directly?

Because glucose oxidase is specific to glucose, and Nelson's reagent detects both, allowing fructose to be calculated by difference.