Urogenital Bacteria

What media are observed in the GI bacteria exercise?

Tomato Juice Agar, EMB agar, SF Broth, and MacConkey agar

Which plate is used to inoculate a TSI slant?

MacConkey agar

What colony is used to inoculate the TSI slant?

An isolated lactose-fermenting colony

Which test is inoculated from a MacConkey colony?

Enterotube

What are all enterics?

Gram-negative, oxidase-negative bacilli

What metabolic characteristic do all enterics share?

Produce acid from glucose fermentation

Which two media are primarily used to identify enterics?

MacConkey agar and TSI agar

Which lactose fermenter produces H₂S?

Citrobacter

Which lactose fermenter is indole positive?

Escherichia

Which lactose fermenter is motile?

Enterobacter

Which lactose fermenter is nonmotile?

Klebsiella

Which non-lactose fermenter produces H₂S and urease?

Proteus

Which organism is urease positive?

Proteus

Which non-lactose fermenter produces H₂S and ferments mannitol?

Salmonella

Which organism ferments mannitol?

Salmonella

Which non-lactose fermenter produces H₂S but does not ferment mannitol?

Edwardsiella

Which organism produces pigment at 25°C?

Serratia

Which organism does not produce pigment at 25°C?

Shigella

Which organism has ornithine decarboxylase?

Morganella

Which organism lacks ornithine decarboxylase?

Providencia

What ingredients are in EMB agar?

Eosin, methylene blue, lactose, sucrose, and peptone

EMB agar is what type of medium?

Selective and differential

What ingredients are in MacConkey agar?

Bile salts, crystal violet, lactose, casein, and gelatin

MacConkey agar is what type of medium?

Selective and differential

What does TSI stand for?

Triple Sugar Iron agar

What sugars are found in TSI agar?

Glucose, lactose, and sucrose

What indicator is found in TSI agar?

Phenol red

What compound detects H₂S production in TSI agar?

Ferrous sulfate (FeSO₄)

What sulfur source is in TSI agar?

Sodium thiosulfate

What is the purpose of the Enterotube?

Rapid identification of enteric bacteria

Which organisms are listed as causes of cystitis?

Escherichia coli, Proteus, and Pseudomonas

E. coli is classified as what type of enteric?

Coliform enteric

Proteus is classified as what type of enteric?

Non-coliform enteric

What media are used in the urogenital tract exercise?

Blood agar and MacConkey agar

Which plate is inoculated with diluted urine?

Blood agar

Which plate is inoculated with undiluted urine?

MacConkey agar

How much saline is used for the urine dilution?

Two tubes containing 0.9 mL saline

What does CLED stand for?

Cystine Lactose Electrolyte Deficient

What does EMB stand for?

Eosin Methylene Blue

Which organism slide is observed during the urogenital tract exercise?

Neisseria gonorrhoeae

What type of urine specimen is collected?

Clean-catch urine specimen

How much urine is added to the first saline tube?

0.1 mL

How much is transferred from saline tube #1 to tube #2?

0.1 mL

How much is transferred from saline tube #2 to the blood agar plate?

0.1 mL

What is used to spread the urine on the blood agar plate?

A spreading rod

At what temperature are urine cultures incubated?

37°C

How is the Uricheck culture inoculated?

Dip the paddle into the urine sample

At what temperature is the Uricheck culture incubated?

37°C